Simão Adriano Prado, Almeida Tássio Málber de Oliveira, Mendonça Vanessa Amaral, Santos Sérgio Antunes, Gomes Wellington Fabiano, Coimbra Cândido Celso, Lacerda Ana Cristina Rodrigues
Integrated Center for Graduate Studies and Research Health (CIPq-Health), Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina, Minas Gerais, Brazil,
Rheumatol Int. 2014 Dec;34(12):1759-63. doi: 10.1007/s00296-014-3016-0. Epub 2014 Apr 20.
The aim of the study was to analyze the concentrations of sTNFR1 and sTNFR2 in both plasma and synovial fluid of patients with primary knee osteoarthritis (OA) and to determine their relationship to self-reported pain, stiffness and physical function. Twenty-seven patients with knee OA and 19 healthy subjects were enrolled in the study. The Western Ontario and McMaster University Osteoarthritis Index questionnaire was used to evaluate self-reported physical function, pain and stiffness. The sTNFR1 and sTNFR2 levels in the plasma and synovial fluid were measured by enzyme-linked immunosorbent assay. The sTNFR1 levels in synovial fluid of OA patients (2,587 ± 66.12 pg/mL) were 2.5-fold higher than in corresponding blood samples and were 1.5-fold higher than in the plasma of healthy controls. The plasma sTNFR2 levels in the patients with knee OA were lower than in healthy controls (2,249 ± 126.3 vs. 2,700 ± 126.3 pg/mL, p < 0.05), and sTNFR2 levels in synovial fluid of knee OA patients (2,021 ± 107.0 pg/mL) were lower than in the plasma of healthy controls. Synovial fluid sTNFR1 levels were negatively correlated with pain and physical function self-reported (r s - 0.6785, p < 0.0001 and r s - 0.4194, p = 0.03, respectively). Synovial fluid sTNFR2 levels were negatively correlated with pain and joint stiffness (r s - 0.5433, p = 0.01 and r s - 0.4249, p = 0.02, respectively). The findings of this study demonstrated the presence of soluble receptors for TNF-alpha, particularly sTNFR1, in the synovial fluid of patients with primary knee OA and the relationship of these receptors with clinical parameters.
本研究旨在分析原发性膝关节骨关节炎（OA）患者血浆和滑液中sTNFR1和sTNFR2的浓度，并确定它们与自我报告的疼痛、僵硬和身体功能之间的关系。27例膝关节OA患者和19名健康受试者参与了本研究。采用西安大略和麦克马斯特大学骨关节炎指数问卷来评估自我报告的身体功能、疼痛和僵硬程度。通过酶联免疫吸附测定法测量血浆和滑液中的sTNFR1和sTNFR2水平。OA患者滑液中的sTNFR1水平（2587±66.12 pg/mL）比相应血样中的高2.5倍，比健康对照者血浆中的高1.5倍。膝关节OA患者的血浆sTNFR2水平低于健康对照者（2249±126.3 vs. 2700±126.3 pg/mL，p<0.05），膝关节OA患者滑液中的sTNFR2水平低于健康对照者血浆中的水平。滑液sTNFR1水平与自我报告的疼痛和身体功能呈负相关（分别为rs - 0.6785，p<0.0001和rs - 0.4194，p = 0.03）。滑液sTNFR2水平与疼痛和关节僵硬呈负相关（分别为rs - 0.5433，p = 0.01和rs - 0.4249，p = 0.02）。本研究结果表明，原发性膝关节OA患者的滑液中存在肿瘤坏死因子-α的可溶性受体，尤其是sTNFR1，以及这些受体与临床参数之间的关系。
