Peebles S E, McNicol A M
University Department of Pathology, Royal Infirmary, Glasgow, U.K.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(4):209-12. doi: 10.1007/BF02899083.
Using a combined silver staining/immunoalkaline phosphatase technique, nucleolar organiser regions (AgNORs) were visualised and quantified in rat anterior and intermediate lobe pituitary corticotrophs following bilateral adrenalectomy or sham surgery. Compared to sham operated animals, the mean number of AgNORs was increased in anterior lobe corticotrophs in adrenalectomized rats and there was a shift to the right in the distribution. At 2 weeks after adrenalectomy, AgNOR numbers were greater than at 6 weeks. AgNOR numbers were also quantified in anterior lobe corticotrophs of intact rats receiving daily intraperitoneal injections of ovine CRF-41 at 50 micrograms/kg, which has been shown to stimulate ACTH release and to produce morphological evidence of increased corticotroph stimulation. CRF-41 did not produce an increase in AgNOR numbers, compared to saline injected controls.
采用银染色/免疫碱性磷酸酶联合技术，对双侧肾上腺切除或假手术后大鼠垂体前叶和中间叶促肾上腺皮质激素细胞中的核仁组织区（AgNORs）进行可视化和定量分析。与假手术动物相比，肾上腺切除大鼠前叶促肾上腺皮质激素细胞中AgNORs的平均数量增加，且分布向右偏移。肾上腺切除术后2周时的AgNOR数量多于术后6周时。还对每天腹腔注射50微克/千克羊CRF-41的完整大鼠前叶促肾上腺皮质激素细胞中的AgNOR数量进行了定量分析，已有研究表明该剂量的CRF-41可刺激促肾上腺皮质激素（ACTH）释放，并产生促肾上腺皮质激素细胞刺激增加的形态学证据。与注射生理盐水的对照组相比，CRF-41并未使AgNOR数量增加。
