[呼吸道合胞病毒肾病大鼠肾组织中乙酰肝素酶的表达及其与蛋白尿的关系]

[Expression of heparanase in kidney of rats with respiratory syncytial virus nephropathy and its relationship with proteinurina].

作者信息

Tao Yu-Hong, Wang Zheng, Zhou Yan-Ru

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2014 Mar;45(2):212-5, 224.

PMID:24749342

Abstract

OBJECTIVE

To explore the role of heparanase in the pathogenesis of respiratory syncytial virus (RSV) nephropathy in rats model.

METHODS

Twenty 150-200 g Sprague-Dawley (SD) rats (n = 5 per group) were inoculated with 6 x 10(6) PFU RSV and sacrificed on days 4, 8, 14 and 28 postinoculation (RSV4, RSV8, RSV14 and RSV29). Five SD rats inoculated with Dulbecco's minimum essential medium were served as normal control. The expression levels of heparanase protein and mRNA in the rat renal tissue of each group were determined by immunohistochemical staining and real-time quantitative RT-PCR respectively. The proteinurina was also measured and then the relationship between the expression level of heparanase and the 24-hour urinary protein was studied.

RESULTS

The rats with RSV nephropathy exhibited higher proteinuria in comparison with normal rats, and the 24-hour urinary protein level was significantly different between each RSV nephropathy group (RSV14 > RSV8 > RSV28 > RSV4, P < 0.05). Compared with normal control, the rats with RSV nephropathy showed up-regulated expression of heparanase protein in glomeruli. The expression levels of heparanase protein in RSV8 and RSV14 group were higher than those in RSV4 and RSV28 group (P < 0.05). There was a linear positive correlation between the expression level of glomerular heparanase protein and the quantity of 24-hour urinary protein (r = 0.783, P < 0.05). Compared with normal control group, the expression levels of heparanase mRNA in the kidney from RSV4, RSV8, RSV14, and RSV28 group were elevated (RSV14 > RSV8 > RSV4 > RSV28 , P < 0.05). There was a linear positive correlation between the expression level of renal heparanase mRNA and the quantity of 24-hour urinary protein (r = 0.725, P < 0.05).

CONCLUSION

The increased expression of heparanase in kidney may be important to the loss of glomerular negative charge in glomerular basement membrane which is involved in the pathogenesis of RSV nephropathy in rats.

摘要

目的

探讨乙酰肝素酶在大鼠呼吸道合胞病毒（RSV）肾病发病机制中的作用。

方法

将20只体重150 - 200 g的Sprague-Dawley（SD）大鼠（每组n = 5只）接种6×10⁶ PFU RSV，并在接种后第4、8、14和28天（RSV4、RSV8、RSV14和RSV28）处死。5只接种杜尔贝科改良伊格尔培养基的SD大鼠作为正常对照。分别采用免疫组化染色和实时定量RT-PCR法检测各组大鼠肾组织中乙酰肝素酶蛋白和mRNA的表达水平。同时检测蛋白尿情况，研究乙酰肝素酶表达水平与24小时尿蛋白之间的关系。

结果

与正常大鼠相比，RSV肾病大鼠蛋白尿水平更高，各RSV肾病组之间24小时尿蛋白水平差异有统计学意义（RSV14 > RSV8 > RSV28 > RSV4，P < 0.05）。与正常对照组相比，RSV肾病大鼠肾小球中乙酰肝素酶蛋白表达上调。RSV8和RSV14组乙酰肝素酶蛋白表达水平高于RSV4和RSV28组（P < 0.05）。肾小球乙酰肝素酶蛋白表达水平与24小时尿蛋白量呈线性正相关（r = 0.783，P < 0.05）。与正常对照组相比，RSV4、RSV8、RSV14和RSV28组大鼠肾脏中乙酰肝素酶mRNA表达水平升高（RSV14 > RSV8 > RSV4 > RSV28，P < 0.05）。肾组织乙酰肝素酶mRNA表达水平与24小时尿蛋白量呈线性正相关（r = 0.725，P < 0.05）。