J Biomed Nanotechnol. 2014 Jun;10(6):1080-90. doi: 10.1166/jbn.2014.1819.
The osteogenic effect of culturing adipose-derived stem cells (ADSCs) on alendronate (Aln)-loaded polycarprolactone (PCL) nanofibrous scaffolds was evaluated by examining alkaline phosphatase (ALP) activity, calcium content, and expression of osteogenic differentiation genes in vitro. The 10% Aln/PCL nanofibrous scaffolds showed more ALP activity, mineralization, and osteocalcin and osteopontin mRNA than the 1% or 5% Aln/PCL nanofibrous scaffolds. The capacity of Aln/PCL nanofibrous scaffolds to regenerate new bone was studied in a rat calvarial defect model. New bone formation in vivo was evaluated by radiography, micro-computed tomography, and histological analysis. At 8 weeks after implantation, Aln/PCL scaffolds had a positive effect on bone regeneration and matrix formation. These results suggested that Aln/PCL nanofibrous scaffolds enhanced the osteogenic differentiation of ADSCs in vitro and bone formation in vivo.
通过检测碱性磷酸酶（ALP）活性、钙含量和骨向分化基因的表达，评估了在载阿仑膦酸钠（Aln）的聚己内酯（PCL）纳米纤维支架上培养脂肪来源干细胞（ADSCs）的成骨作用。10%Aln/PCL 纳米纤维支架的 ALP 活性、矿化程度以及骨钙素和骨桥蛋白 mRNA 的表达均高于 1%或 5%Aln/PCL 纳米纤维支架。在大鼠颅骨缺损模型中研究了 Aln/PCL 纳米纤维支架再生新骨的能力。通过 X 射线、微计算机断层扫描和组织学分析评估体内新骨形成。植入后 8 周，Aln/PCL 支架对骨再生和基质形成有积极影响。这些结果表明，Aln/PCL 纳米纤维支架增强了 ADSCs 的体外成骨分化和体内骨形成。
