Covey D C, Chang C C, Laurence K A
Department of Biological Sciences, University of Idaho, Moscow.
Am J Reprod Immunol. 1989 Jan;19(1):17-20. doi: 10.1111/j.1600-0897.1989.tb00542.x.
Corynebacterium parvum and Bordetella pertussis were compared with Freund's complete adjuvant (FCA) for their abilities to potentiate the immune response to haptenic beta-human chorionic gonadotropin covalently coupled to an atoxic 54,000-molecular-weight fragment of tetanus toxin (beta-hCG-TTII). The ability of each adjuvant to enhance production of antibodies to hCG in rabbits was measured by 125I-hCG radioimmunoassay. At sera dilutions of 1:10,000, analysis of variance for the 8-week postimmunization course showed that the mean 125I-hCG binding capacities of the C. parvum group was significantly greater overall than the B. pertussis group (P = .0002) and that the FCA-treated group had the greatest binding capacity overall (P less than .018). The mean binding capacities at 1:40,000 dilution again showed the FCA-treated group to have significantly higher anti-hCG titers overall (P less than .0015), with C. parvum potentiating a greater overall antibody response than B. pertussis (P = .001). These results indicate that FCA is the most efficacious of the three tested adjuvants in potentiating antibody production to the hapten component of beta-hCG-TTII. C. parvum was also effective at promoting an anti-beta-hCG response, although not to the same degree as FCA. B. pertussis had only minimal potentiating effect compared to FCA or C. parvum.
将微小棒状杆菌和百日咳博德特氏菌与弗氏完全佐剂（FCA）进行比较，以评估它们增强对半抗原β-人绒毛膜促性腺激素与破伤风毒素无毒54,000分子量片段共价偶联物（β-hCG-TTII）免疫反应的能力。通过125I-hCG放射免疫测定法测量每种佐剂增强兔体内抗hCG抗体产生的能力。在免疫后8周的过程中，在血清稀释度为1:10,000时，方差分析表明，微小棒状杆菌组的平均125I-hCG结合能力总体上显著高于百日咳博德特氏菌组（P = 0.0002），且FCA处理组的总体结合能力最强（P < 0.018）。在1:40,000稀释度下的平均结合能力再次表明，FCA处理组的总体抗hCG滴度显著更高（P < 0.0015），微小棒状杆菌增强的总体抗体反应比百日咳博德特氏菌更强（P = 0.001）。这些结果表明，在增强针对β-hCG-TTII半抗原成分的抗体产生方面，FCA是三种测试佐剂中最有效的。微小棒状杆菌在促进抗β-hCG反应方面也有效，尽管程度不如FCA。与FCA或微小棒状杆菌相比，百日咳博德特氏菌的增强作用最小。
