在 HCV 单感染和 HIV/HCV 合并感染患者中，肝组织 HMOX1 的表达与 Bach-1 和 miR-122 呈正相关。
Hepatic HMOX1 expression positively correlates with Bach-1 and miR-122 in patients with HCV mono and HIV/HCV coinfection.
机构信息
Department of Infectious Diseases and Hepatology, Medical University of Lodz, Łódź, Poland.
Central Laboratory, Medical University of Lodz, Łódź, Poland.
出版信息
PLoS One. 2014 Apr 21;9(4):e95564. doi: 10.1371/journal.pone.0095564. eCollection 2014.
AIM
To analyze the expression of HMOX1 and miR-122 in liver biopsy samples obtained from HCV mono-and HIV/HCV co-infected patients in relation to selected clinical parameters, histological examination and IL-28B polymorphism as well as to determine whether HMOX1 expression is dependent on Bach-1.
MATERIALS AND METHODS
The study group consisted of 90 patients with CHC: 69 with HCV mono and 21 with HIV/HCV co-infection. RT-PCR was used in the analysis of HMOX1, Bach-1 and miR-122 expression in liver biopsy samples and in the assessment of IL-28B single-nucleotide polymorphism C/T (rs12979860) in the blood. Moreover in liver biopsy samples an analysis of HO-1 and Bach-1 protein level by Western Blot was performed.
RESULTS
HCV mono-infected patients, with lower grading score (G<2) and higher HCV viral load (>600000 IU/mL) demonstrated higher expression of HMOX1. In patients with HIV/HCV co-infection, the expression of HMOX1 was lower in patients with lower lymphocyte CD4 count and higher HIV viral load. IL28B polymorphism did not affect the expression of either HMOX1 or miR-122. Higher HMOX1 expression correlated with higher expression of Bach-1 (Spearman's ρ = 0.586, p = 0.000001) and miR-122 (Spearman's ρ = 0.270, p = 0.014059).
CONCLUSIONS
HMOX1 and miR-122 play an important role in the pathogenesis of CHC in HCV mono-and HIV/HCV co-infected patients. Reduced expression of HMOX1 in patients with HIV/HCV co-infection may indicate a worse prognosis in this group. Our results do not support the importance of Bach-1 in repression of HMOX1 in patients with chronic hepatitis C.
目的
分析 HCV 单感染和 HIV/HCV 共感染患者肝活检样本中 HMOX1 和 miR-122 的表达与选定的临床参数、组织学检查和 IL-28B 多态性的关系，并确定 HMOX1 的表达是否依赖于 Bach-1。
材料与方法
研究组包括 90 例 CHC 患者：69 例 HCV 单感染和 21 例 HIV/HCV 共感染。使用 RT-PCR 分析肝活检样本中 HMOX1、Bach-1 和 miR-122 的表达，并检测血液中 IL-28B 单核苷酸多态性 C/T(rs12979860)。此外，通过 Western Blot 分析肝活检样本中 HO-1 和 Bach-1 蛋白水平。
结果
HCV 单感染患者，分级评分较低（G<2）和 HCV 病毒载量较高（>600000 IU/mL），HMOX1 表达较高。HIV/HCV 共感染患者中，淋巴细胞 CD4 计数较低和 HIV 病毒载量较高的患者 HMOX1 表达较低。IL28B 多态性不影响 HMOX1 或 miR-122 的表达。较高的 HMOX1 表达与 Bach-1 表达升高相关（Spearman's ρ=0.586，p=0.000001）和 miR-122（Spearman's ρ=0.270，p=0.014059）。
结论
HMOX1 和 miR-122 在 HCV 单感染和 HIV/HCV 共感染患者 CHC 的发病机制中发挥重要作用。HIV/HCV 共感染患者 HMOX1 表达降低可能表明该组预后较差。我们的结果不支持 Bach-1 在慢性丙型肝炎患者中抑制 HMOX1 表达的重要性。
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