Asmari Abdulrahman Al, Omani Saud Al, Otaibi Malfi Al, Abdulaaly Abdul-Aziz Al, Elfaki Ibrahim, Yahya Khalid Al, Arshaduddin Mohammed
Research Center, Prince Sultan Military Medical City Riyadh, Saudi Arabia.
Department of Surgery, Prince Sultan Military Medical City Riyadh, Saudi Arabia.
Int J Clin Exp Med. 2014 Mar 15;7(3):586-96. eCollection 2014.
Minocycline (MCN), a semi-synthetic tetracycline derivative possesses pleiotropic effects and provides protection against a number of disease models. However its effect on gastric ulcers has not been studied. The present investigation was undertaken, to study the gastro-protective potential of MCN in experimentally induced gastric ulcers in rats. MCN (10, 30, 100 mg/Kg) was tested for gastric secretion and antiulcer activity in different groups of Wistar rats. Gastric secretion and acidity studies were performed in pylorus ligated rats while indices of gastric ulcers were measured in ethanol (1 ml-100%) and indomethacin (30 mg/kg), induced gastric ulcers. Histological changes and the levels of gastric wall mucus, malondialdehyde (MDA), non-protein sulfhydryl (NP-SH), and myeloperoxidase (MPO), were used to assess ethanol induced gastric mucosal injuries. Exposure of rats to ulcerogens resulted in gastric mucosal injury and a significant increase in the indices of ulcer. MCN conferred a protective effect against ethanol, and indomethacin induced gastric mucosal injuries. Treatment with MCN, resulted in a significant decrease in the amount of gastric secretion, and total acidity and significantly (P<0.001), reduced the gastric lesions induced by ethanol and indomethacin. MCN also significantly attenuated the ethanol induced reduction in the levels of gastric wall mucus, and NP-SH (P<0.001). The histological changes and the increased MDA and MPO activity were also significantly (P<0.001) inhibited by MCN. Minocycline showed significant antiulcer and gastroprotective activity against experimentally induced gastric ulcers. The gastroprotective effects of minocycline may be due to its anti-secretory, antioxidant and anti inflammatory action.
米诺环素（MCN）是一种半合成四环素衍生物，具有多种作用，并能对多种疾病模型起到保护作用。然而，其对胃溃疡的影响尚未得到研究。本研究旨在探讨米诺环素对大鼠实验性胃溃疡的胃保护潜力。对不同组的Wistar大鼠测试了米诺环素（10、30、100毫克/千克）的胃分泌和抗溃疡活性。在幽门结扎的大鼠中进行胃分泌和酸度研究，同时在乙醇（1毫升-100%）和吲哚美辛（30毫克/千克）诱导的胃溃疡中测量胃溃疡指标。利用组织学变化以及胃壁黏液、丙二醛（MDA）、非蛋白巯基（NP-SH）和髓过氧化物酶（MPO）的水平来评估乙醇诱导的胃黏膜损伤。将大鼠暴露于致溃疡物质会导致胃黏膜损伤以及溃疡指标显著增加。米诺环素对乙醇和吲哚美辛诱导的胃黏膜损伤具有保护作用。用米诺环素治疗后，胃分泌量和总酸度显著降低（P<0.001），并显著减轻了乙醇和吲哚美辛诱导的胃损伤。米诺环素还显著减轻了乙醇诱导的胃壁黏液和NP-SH水平的降低（P<0.001）。米诺环素还显著抑制了组织学变化以及MDA和MPO活性的增加（P<0.001）。米诺环素对实验性诱导的胃溃疡显示出显著的抗溃疡和胃保护活性。米诺环素的胃保护作用可能归因于其抗分泌、抗氧化和抗炎作用。
