Al Asmari Abdulrahman, Al Shahrani Hamoud, Al Masri Nasser, Al Faraidi Ahmed, Elfaki Ibrahim, Arshaduddin Mohammed
Research Center, Prince Sultan Military Medical City, Riyadh, Saudi Arabia.
Department of Ophthalmology, Prince Sultan Military Medical City, Riyadh, Saudi Arabia.
Toxicol Rep. 2015 Dec 12;3:105-113. doi: 10.1016/j.toxrep.2015.11.001. eCollection 2016.
Vanillin is commonly used as an additive in food, medicine and cosmetics, but its effect has not yet been studied in gastric injury. Therefore the effect of vanillin was studied in experimental gastric ulcer. Gastric secretion and acidity were studied in pylorus ligated rats. Ulcer index, levels of gastric mucus, malondialdehyde (MDA), myeloperoxidase activity (MPO), expression of nuclear factor kappa B (NF-κB) p65, and histopathological changes were determined in ethanol induced gastric ulcer. Pre treatment with vanillin significantly reduced gastric secretion ( < 0.001) and acidity ( < 0.0001) and gastric ulcer index scores ( < 0.001). and augmented the gastric mucosal defense. Vanillin significantly restored the depleted gastric wall mucus levels ( < 0.0001) induced by ethanol and also significantly attenuated ethanol induced inflammation and oxidative stress by the suppression of gastric MPO activity ( < 0.001), reducing the expression of NF-κB p65 and the increased MDA levels ( < 0.001). Vanillin was also effective in alleviating the damage to the histological architecture and the activation of mast cells induced by ethanol. Together the results of this study highlight the gastroprotective activity of vanillin in gastric ulcers of rats through multiple actions that include inhibition of gastric secretion and acidity, reduction of inflammation and oxidative stress, suppression of expression of NF-κB, and restoration of the histological architecture.
香草醛常用于食品、医药和化妆品中作为添加剂，但其对胃损伤的影响尚未得到研究。因此，研究了香草醛在实验性胃溃疡中的作用。对幽门结扎大鼠的胃分泌和酸度进行了研究。在乙醇诱导的胃溃疡中测定溃疡指数、胃黏液水平、丙二醛（MDA）、髓过氧化物酶活性（MPO）、核因子κB（NF-κB）p65的表达以及组织病理学变化。香草醛预处理显著降低了胃分泌（<0.001）、酸度（<0.0001）和胃溃疡指数评分（<0.001），并增强了胃黏膜防御功能。香草醛显著恢复了乙醇诱导的胃壁黏液水平降低（<0.0001），还通过抑制胃MPO活性（<0.001）、降低NF-κB p65的表达和升高的MDA水平（<0.001），显著减轻了乙醇诱导的炎症和氧化应激。香草醛还能有效减轻乙醇诱导的组织学结构损伤和肥大细胞活化。本研究结果共同突出了香草醛通过多种作用对大鼠胃溃疡的胃保护活性，这些作用包括抑制胃分泌和酸度、减轻炎症和氧化应激、抑制NF-κB表达以及恢复组织学结构。
