Pan Xue-Qiang, Wu Yan-Chuan, Gong Mu-Xin, Xu Yong-Song, Wang Zhi-Min, Zhang Qi-Wei, Shang Ya-Wen, Lu Xu-Ran, Song Ya-Fang
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(1):126-33.
Wuzhuyu Tang is a classical formula for treating migraine, but its' pharmacological ingredients is unclear yet. Present study employed the everted intestinal sac model to collect the absorption samples of 10 kinds of Wuzhuyu decoction, and then analyzed the contents of 9 ingredients in Wuzhuyu Tang and absorption samples quantitatively or semi-quantitatively by HPLC-DAD method. Reserpine was used to establish the mice model of migraine, and then the contents and activities of 5-hydroxytryptamine, noradrenaline, dopamine, nitric oxide and nitricoxide synthase in brain tissues and serums were determined respectively after oral administration of Wuzhuyu Tang. Using the partial least squares regression method to correlate the total absorption quantity of 9 ingredients and pharmacodynamics. The result shows that limocitrin-3-O-beta-D-glucoside, ginsenoside Rg1 and Rb1, rutaevine, limonin, evodiamine and rutaecarpine are the main ingredients influenced the effects in absorption samples in everted intestinal sacs, especially ginsenoside Rg1, rutaevine, evodiamine and rutaecarpine among them have obvious improving effects to most pharmacodynamics index, might be the pharmacological ingredients influenced the therapeutical effects of Wuzhuyu Tang treating migraine.
吴茱萸汤是治疗偏头痛的经典方剂，但其药理成分尚不清楚。本研究采用外翻肠囊模型收集10种吴茱萸汤的吸收样品，然后用HPLC-DAD法对吴茱萸汤及吸收样品中的9种成分进行定量或半定量分析。用利血平建立小鼠偏头痛模型，口服吴茱萸汤后分别测定脑组织和血清中5-羟色胺、去甲肾上腺素、多巴胺、一氧化氮及一氧化氮合酶的含量和活性。采用偏最小二乘回归法将9种成分的总吸收量与药效学进行关联。结果表明，柚皮素-3-O-β-D-葡萄糖苷、人参皂苷Rg1和Rb1、吴茱萸次碱、柠檬苦素、吴茱萸碱和吴茱萸次酸是影响外翻肠囊中吸收样品效果的主要成分，其中人参皂苷Rg1、吴茱萸次碱、吴茱萸碱和吴茱萸次酸对多数药效学指标有明显改善作用，可能是影响吴茱萸汤治疗偏头痛疗效的药理成分。
