[吴茱萸汤吸收成分快速查找的外翻肠囊法]

[Everted intestinal sac method for quick finding absorption ingredients of Wuzhuyu decoction].

作者信息

Gong Muxin, Wang Yaxun, Song Yafang, Wang Zhimin, Zhang Qiwei, Wang Weihao, Zhu Jingjing

机构信息

School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Jun;35(11):1399-404.

PMID:20822007

Abstract

OBJECTIVE

To establish a method for quick finding the absorption ingredients of Wuzhuyu decoction in order to select the index to control its quality.

METHOD

The absorption of three concentration of Wuzhuyu decotion was investigated with the in vitro-everted intestinal sac model. The intestinal bag fluid of jejunum and ileum were collected in different time and the eight ingredients, which were evodiamine (Ev), rutaecarpine (Ru), limonin (Li), ginsenoside-Rb1, -Rg1, -Re (Rb1, Rg1, Re), isorhamnetin-3-O-beta-D-glucosyl(6''-->1'")-alpha-L-rhamnoside (Irs)and 6-gingerol (6-Gi), were detected by HPLC as the represent constituents in samples.

RESULT

Eight ingredients except Ru in samples could be detected, but Ev could not be detected in high concentration samples. The ratios between absorption ingredients were different from in Wuzhuyu decotion.

CONCLUSION

The in vitro-everted intestinal sac canc absorb the ingredients of Wuzhuyu decotion selectivity. Compare with the ileum, the jejunum can provide the more absorption information and faster, the best test time is 60-90 min.

摘要

目的

建立快速筛选吴茱萸汤吸收成分的方法，以选择其质量控制指标。

方法

采用体外翻肠囊模型考察吴茱萸汤3种浓度的吸收情况。于不同时间收集空肠和回肠肠囊液，采用高效液相色谱法检测吴茱萸次碱（Ev）、吴茱萸碱（Ru）、柠檬苦素（Li）、人参皂苷-Rb1、-Rg1、-Re（Rb1、Rg1、Re）、异鼠李素-3-O-β-D-葡萄糖基（6''→1'")-α-L-鼠李糖苷（Irs）和6-姜辣素（6-Gi）这8种成分，作为样品中的代表性成分。