Pan Xueqiang, Wang Manyuan, Wu Yanchuan, Lu Xuran, Shang Yawen, Xu Yongsong, Zhai Yongsong, Li Jing, Li Zhaoxia, Gong Muxin
Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, P.R. China.
Central Laboratory, Xuanwu Hospital of Capital Medical University, Beijing 100069, P.R. China.
Mol Med Rep. 2015 Jul;12(1):1524-34. doi: 10.3892/mmr.2015.3506. Epub 2015 Mar 18.
Wuzhuyu decoction is a traditional Chinese medicine used for the effective treatment of migraines, termed 'Jueyin headache', in China. However, there have been few investigations to clarify the composition of Wuzhuyu decoction for the treatment of migraines. In the present study, 10 types of Wuzhuyu decoction were analyzed by chromatograms. 5-hydroxytryptamine (5-HT)-depletion mouse models of migraine were prepared by subcutaneous injection of reserpine and placement of autologous blood clots in the cerebral cortex. The levels of 5-HT, noradrenaline (NE), dopamine (DA), nitric oxide (NO) and nitric oxide synthase (NOS) in the brain tissues and sera of the mice were determined. The ingredients and pharmacodynamic indices of the Wuzhuyu decoctions were analyzed using spectral efficiency association by partial least squares regression. The levels of 5-HT, NE and DA in the mouse brain tissues were reduced to 337.785 ± 84.504, 171.173 ± 65.172 and 242.075 ± 158.621 mg/g brain tissue, respectively. The level of NO in the brain tissues increased to 0.425 ± 0.184 µmol/g protein and the activities of NOS in the brain tissues and sera increased to 0.719 ± 0.477 U/mg and 50.688 ± 8.132 U/ml, respectively. Regarding the ingredients of the Wuzhuyu decoction, those with significant regression coefficients were ginsenoside-Rg1, Re, Rb1, rutaevine (Rv), limonin (Li), evodiamine (Ev), rutaecarpine (Ru) and substance X (awaiting identification). Rg1, Re, Rb1, Rv, Li, Ev, Ru and X in the Wuzhuyu decoction were observed to yield the pharmacological effects, whereas Rb1, Rv and Ev were important in index improvement.
吴茱萸汤是一种传统中药，在中国用于有效治疗偏头痛，即所谓的“厥阴头痛”。然而，关于吴茱萸汤治疗偏头痛的成分，此前鲜有研究进行阐明。在本研究中，通过色谱图对10种吴茱萸汤进行了分析。通过皮下注射利血平并在大脑皮层放置自体血凝块，制备了5-羟色胺（5-HT）耗竭型偏头痛小鼠模型。测定了小鼠脑组织和血清中5-HT、去甲肾上腺素（NE）、多巴胺（DA）、一氧化氮（NO）和一氧化氮合酶（NOS）的水平。采用偏最小二乘回归的光谱效率关联分析吴茱萸汤的成分和药效学指标。小鼠脑组织中5-HT、NE和DA的水平分别降至337.785±84.504、171.173±65.172和242.075±158.621mg/g脑组织。脑组织中NO水平升至0.425±0.184µmol/g蛋白，脑组织和血清中NOS活性分别升至0.719±0.477U/mg和50.688±8.132U/ml。关于吴茱萸汤的成分，具有显著回归系数的成分有人参皂苷-Rg1、Re、Rb1、吴茱萸次碱（Rv）、柠檬苦素（Li）、吴茱萸碱（Ev）、吴茱萸次酸（Ru）和物质X（待鉴定）。观察到吴茱萸汤中的Rg1、Re、Rb1、Rv、Li、Ev、Ru和X产生了药理作用，而Rb1、Rv和Ev对指标改善很重要。
