Steiner H J, Schmid K W, Fischer-Colbrie R, Sperk G, Winkler H
Department of Pathology, University of Innsbruck, Austria.
Histochemistry. 1989;91(6):473-7. doi: 10.1007/BF00492518.
The co-localization of various antigens in rat chromaffin granules was investigated by the immunogold staining procedure. In ultrathin serial sections staining of chromaffin granules was obtained with antisera against chromogranin A, chromogranin B, secretogranin II and neuropeptide Y. These results indicated that these antigens are costored within chromaffin granules. To further corroborate this point a double immunogold staining procedure was used. This method unequivocally established that chromogranin A, chromogranin B, secretogranin II and neuropeptide Y are co-localized in the same chromaffin granules. These results are relevant for studies demonstrating changes in the level of these peptides in adrenal medulla. The co-localization makes it likely that such changes lead to a different relative composition of the secretory quanta of chromaffin granules.
通过免疫金染色法研究了大鼠嗜铬颗粒中各种抗原的共定位。在超薄连续切片中，用抗嗜铬粒蛋白A、嗜铬粒蛋白B、分泌粒蛋白II和神经肽Y的抗血清对嗜铬颗粒进行染色。这些结果表明这些抗原共同储存在嗜铬颗粒中。为了进一步证实这一点，采用了双重免疫金染色法。该方法明确证实嗜铬粒蛋白A、嗜铬粒蛋白B、分泌粒蛋白II和神经肽Y共定位于同一嗜铬颗粒中。这些结果与证明肾上腺髓质中这些肽水平变化的研究相关。共定位使得这种变化可能导致嗜铬颗粒分泌量子的相对组成不同。
