Settleman J, Nolan J, Angeletti R H
J Biol Chem. 1985 Feb 10;260(3):1641-4.
Chromogranin is the major soluble protein of the adrenal medulla chromaffin granule and is secreted upon nervous stimulation. Using antisera to pure chromogranin in immunoblotting procedures, we show that chromogranin is the major integral membrane protein as well. Extraction of chromaffin granule membranes with low salt, high salt, chelating agents, or calcium-containing solutions does not remove the chromogranin from the membranes. The membrane form of chromogranin can be purified on a C-18 semi-preparative column using high pressure liquid chromatography. Amino-terminal sequence data indicate that the membrane and soluble forms of chromogranin are identical or very similar.
嗜铬粒蛋白是肾上腺髓质嗜铬颗粒的主要可溶性蛋白，在神经刺激时分泌。在免疫印迹实验中使用针对纯嗜铬粒蛋白的抗血清，我们发现嗜铬粒蛋白也是主要的整合膜蛋白。用低盐、高盐、螯合剂或含钙溶液提取嗜铬颗粒膜并不能将嗜铬粒蛋白从膜上除去。嗜铬粒蛋白的膜形式可以使用高压液相色谱在C-18半制备柱上进行纯化。氨基末端序列数据表明，嗜铬粒蛋白的膜形式和可溶性形式相同或非常相似。
