Majane E A, Alho H, Kataoka Y, Lee C H, Yang H Y
Endocrinology. 1985 Sep;117(3):1162-8. doi: 10.1210/endo-117-3-1162.
Neuropeptide Y (NPY) distribution in bovine adrenal glands was studied by RIA and immunohistochemical technique. NPY content (picomoles per mg protein +/- SEM) of chromaffin cells, medulla, cortex, and whole glands was 4.2 +/- 0.16, 2.7 +/- 0.28, 0.19 +/- 0.02, and 0.94 +/- 0.14, respectively, while the chromaffin granule NPY content was 53. Immunohistochemically, NPY immunoreactivity was detected in norepinephrine containing chromaffin cells and also in nerve fibers crossing through adrenal cortex and medulla. NPY and enkephalin immunoreactivities were found in distinct chromaffin cells. Biochemical characterization by HPLC revealed two major NPY immunoreactive peptides. The most abundant molecular form was identified as authentic NPY.
采用放射免疫分析和免疫组织化学技术研究了神经肽Y（NPY）在牛肾上腺中的分布。嗜铬细胞、髓质、皮质和整个腺体的NPY含量（每毫克蛋白质皮摩尔数±标准误）分别为4.2±0.16、2.7±0.28、0.19±0.02和0.94±0.14，而嗜铬颗粒中的NPY含量为53。免疫组织化学检测发现，含去甲肾上腺素的嗜铬细胞以及穿过肾上腺皮质和髓质的神经纤维中均有NPY免疫反应性。在不同的嗜铬细胞中发现了NPY和脑啡肽免疫反应性。通过高效液相色谱进行的生化特性分析显示有两种主要的NPY免疫反应性肽。最丰富的分子形式被鉴定为天然NPY。
