Leung Frankie Chi-Ming, Tam Anthony Yiu-Yan, Au Vonika Ka-Man, Li Mei-Jin, Yam Vivian Wing-Wah
Institute of Molecular Functional Materials (Areas of Excellence Scheme, University Grants Committee (Hong Kong)) and Department of Chemistry, The University of Hong Kong , Pokfulam Road, Hong Kong SAR, P. R. China.
ACS Appl Mater Interfaces. 2014 May 14;6(9):6644-53. doi: 10.1021/am500350c. Epub 2014 Apr 22.
A number of ruthenium(II) and rhenium(I) bipyridine complexes functionalized with lipoic acid moieties have been synthesized and characterized. Functionalization of gold nanoparticles with these chromophoric ruthenium(II) and rhenium(I) complexes has resulted in interesting supramolecular assemblies with Förster resonance energy transfer (FRET) properties that could be modulated via esterase hydrolysis. The luminescence of the metal complex chromophores was turned on upon cleavage of the ester bond linkage by esterase to reduce the efficiency of FRET quenching. The prepared nanoassembly conjugates have been characterized by transmission electron microscopy (TEM), energy-dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), UV-visible spectroscopy, and emission spectroscopy. The quenching mechanism has also been studied by transient absorption and time-resolved emission decay measurements. The FRET efficiencies were found to vary with the nature of the chromophores and the length of the spacer between the donor (transition metal complexes) and the acceptor (gold nanoparticles).
已合成并表征了多种用硫辛酸部分功能化的钌（II）和铼（I）联吡啶配合物。用这些发色性钌（II）和铼（I）配合物对金纳米颗粒进行功能化，得到了具有福斯特共振能量转移（FRET）特性的有趣超分子组装体，其可通过酯酶水解进行调节。当酯酶裂解酯键连接以降低FRET猝灭效率时，金属配合物发色团的发光被开启。所制备的纳米组装共轭物已通过透射电子显微镜（TEM）、能量色散X射线分析（EDX）、傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、紫外可见光谱和发射光谱进行了表征。还通过瞬态吸收和时间分辨发射衰减测量研究了猝灭机制。发现FRET效率随发色团的性质以及供体（过渡金属配合物）和受体（金纳米颗粒）之间间隔基的长度而变化。
