Zhang Jin, Liu Yang, Walker William B, Dong Shuang-Lin, Wang Gui-Rong
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing.
Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Insect Sci. 2015 Mar;22(3):399-408. doi: 10.1111/1744-7917.12131. Epub 2014 Jul 10.
Sensory neuron membrane proteins (SNMPs), which are located on the dendritic membrane of olfactory sensory neurons (OSNs), are proposed to be associated with odor reception in insects. Recent studies have demonstrated that SNMP1 is essential for electrophysiological responses of OSNs to the sex pheromone, cis-vaccenyl acetate (cVA) in Drosophila melanogaster. To investigate the function of Lepidoptera SNMPs, we cloned two SNMP genes, SlituSNMP1 and SltiuSNMP2, from Spodoptera litura (Lepidoptera: Noctuidae). Sequence alignment and phylogenetic analysis showed that both genes bear the general characteristics of SNMPs, including six conserved cysteine residues and two transmembrane domains. Further expression profile experiments showed that SlituSNMP1 is mainly expressed in the antenna, while SlituSNMP2 is broadly expressed in various tissues. By in situ hybridization experiments, it was found that SlituSNMP1 expressing cells are surrounded by the SlituSNMP2 expressing cells in the pheromone sensitive sensilla, suggesting different functions of the two SNMPs in insect olfaction.
感觉神经元膜蛋白（SNMPs）位于嗅觉感觉神经元（OSNs）的树突膜上，被认为与昆虫的气味接收有关。最近的研究表明，SNMP1对于黑腹果蝇中OSNs对性信息素顺式-乙酸vaccenyl酯（cVA）的电生理反应至关重要。为了研究鳞翅目SNMPs的功能，我们从斜纹夜蛾（鳞翅目：夜蛾科）中克隆了两个SNMP基因，即SlituSNMP1和SltiuSNMP2。序列比对和系统发育分析表明，这两个基因都具有SNMPs的一般特征，包括六个保守的半胱氨酸残基和两个跨膜结构域。进一步的表达谱实验表明，SlituSNMP1主要在触角中表达，而SlituSNMP2在各种组织中广泛表达。通过原位杂交实验发现，在信息素敏感的感器中，表达SlituSNMP1的细胞被表达SlituSNMP2的细胞包围，这表明这两种SNMPs在昆虫嗅觉中具有不同的功能。
