Torres-Huerta Brenda, Segura-León Obdulia L, Aragón-Magadan Marco A, González-Hernández Héctor
Entomology and Acarology Program, Colegio de Postgraduados Campus Montecillo, Mexico-Texcoco, km. 36.5, C. P. 56230, Montecillo, State of Mexico, Mexico.
Sci Rep. 2020 Nov 26;10(1):20695. doi: 10.1038/s41598-020-77144-5.
The round-headed pine beetle Dendroctonus adjunctus, whose dispersion and colonization behaviors are linked to a communication system mediated by semiochemicals, is one of the five most critical primary pests in forest ecosystems in Mexico. This study provides the first head transcriptome analysis of D. adjunctus and the identification of the nonreceptor olfactory genes involved in the perception of odors. De novo assembly yielded 44,420 unigenes, and GO annotations were similar to those of antennal transcriptomes of other beetle species, which reflect metabolic processes related to smell and signal transduction. A total of 36 new transcripts of nonreceptor olfactory genes were identified, of which 27 encode OBPs, 7 encode CSPs, and 2 encode SNMP candidates, which were subsequently compared to homologous proteins from other bark beetles and Coleoptera species by searching for sequence motifs and performing phylogenetic analyses. Our study provides information on genes encoding nonreceptor proteins in D. adjunctus and broadens the knowledge of olfactory genes in Coleoptera and bark beetle species, and will help to understand colonization and aggregation behaviors for the development of tools that complement management strategies.
圆头松小蠹（Dendroctonus adjunctus）的扩散和定殖行为与一种由信息化学物质介导的通讯系统相关，它是墨西哥森林生态系统中五种最关键的主要害虫之一。本研究首次对圆头松小蠹进行了头部转录组分析，并鉴定了参与气味感知的非受体嗅觉基因。从头组装产生了44420个单基因，基因本体（GO）注释与其他甲虫物种的触角转录组注释相似，反映了与嗅觉和信号转导相关的代谢过程。共鉴定出36个非受体嗅觉基因的新转录本，其中27个编码气味结合蛋白（OBPs），7个编码化学感受蛋白（CSPs），2个编码感觉神经元膜蛋白（SNMP）候选蛋白，随后通过搜索序列基序和进行系统发育分析，将它们与其他小蠹虫和鞘翅目物种的同源蛋白进行了比较。我们的研究提供了圆头松小蠹中编码非受体蛋白的基因信息，拓宽了对鞘翅目和小蠹虫物种嗅觉基因的认识，并将有助于理解定殖和聚集行为，以开发补充管理策略的工具。
