Huang Xinglong, Liu Lu, Fang Yiqing, Feng Jinian
Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China (
J Econ Entomol. 2016 Aug;109(4):1907-13. doi: 10.1093/jee/tow098. Epub 2016 Jun 21.
In insects, sensory neuron membrane proteins (SNMPs) are critical peripheral olfactory proteins and highly promote the sensitivity of pheromone detection. In this study, we cloned an SNMP transcript (CpomSNMP2, GenBank KU302714) from the antennae of the codling moth Cydia pomonella (L.) Its open reading frame is 1,575 bp and it encodes a protein with 524 amino acids. CpomSNMP2 contains two putative transmembrane domains and has a large extracellular loop. Phylogenetic analysis showed that CpomSNMP2 is clustered into the group of previously characterized lepidopteron SNMP2s. Expression levels of CpomSNMP2 were significantly higher in antennae of both males and females than in tissues from the thoraxes, abdomens, legs, and wings. CpomSNMP2 was distributed in sensilla trichodea of both males and females, but only in sensilla chaetica of males. This study provides evidence for olfactory roles of CpomSNMP2 in this moth.
在昆虫中，感觉神经元膜蛋白（SNMPs）是关键的外周嗅觉蛋白，能极大地提高信息素检测的灵敏度。在本研究中，我们从苹果蠹蛾Cydia pomonella（L.）的触角中克隆了一个SNMP转录本（CpomSNMP2，GenBank登录号KU302714）。其开放阅读框为1575 bp，编码一个含有524个氨基酸的蛋白质。CpomSNMP2包含两个假定的跨膜结构域，并有一个大的细胞外环。系统发育分析表明，CpomSNMP2聚类到先前已鉴定的鳞翅目SNMP2s组中。CpomSNMP2在雄性和雌性触角中的表达水平均显著高于胸部、腹部、腿部和翅膀的组织。CpomSNMP2分布于雄性和雌性的毛形感器中，但仅分布于雄性的刺形感器中。本研究为CpomSNMP2在这种蛾类中的嗅觉作用提供了证据。
