• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody.天冬氨酸包覆的金纳米颗粒的合成及其与FVIII蛋白和FVIII抗体的偶联
Indian J Clin Biochem. 2014 Apr;29(2):154-60. doi: 10.1007/s12291-013-0323-2. Epub 2013 Apr 2.
2
Synthesis and capping of water-dispersed gold nanoparticles by an amino acid: bioconjugation and binding studies.氨基酸对水分散性金纳米颗粒的合成与封端：生物共轭及结合研究
J Colloid Interface Sci. 2008 Jul 15;323(2):247-54. doi: 10.1016/j.jcis.2008.04.043. Epub 2008 Apr 24.
3
Extinction, emission, and scattering spectroscopy of 5-50 nm citrate-coated gold nanoparticles: An argument for curvature effects on aggregation.5-50nm 柠檬酸金纳米粒子的消光、发射和散射光谱：对聚集过程中曲率效应的论证。
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Mar 15;175:100-109. doi: 10.1016/j.saa.2016.11.052. Epub 2016 Dec 5.
4
Core-size regulated aggregation/disaggregation of citrate-coated gold nanoparticles (5-50nm) and dissolved organic matter: Extinction, emission, and scattering evidence.核尺寸调控的柠檬酸盐包覆金纳米颗粒（5 - 50纳米）与溶解有机物的聚集/解聚：消光、发射和散射证据。
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:415-426. doi: 10.1016/j.saa.2017.08.019. Epub 2017 Aug 10.
5
Gold Nanoparticles Conjugated with Glycopeptides for Lectin Detection and Imaging on Cell Surface.糖肽偶联金纳米颗粒用于细胞表面凝集素的检测与成像
Protein Pept Lett. 2018;25(1):84-89. doi: 10.2174/0929866525666171218124434.
6
Poly (Vinyl Alcohol) Assisted Synthesis and Anti-Solvent Precipitation of Gold Nanoparticles.聚乙烯醇辅助合成及反溶剂沉淀法制备金纳米颗粒
Nanomaterials (Basel). 2020 Nov 27;10(12):2359. doi: 10.3390/nano10122359.
7
Colloidal stability of gold nanoparticles modified with thiol compounds: bioconjugation and application in cancer cell imaging.巯基化合物修饰的金纳米粒子的胶体稳定性：生物缀合及其在癌细胞成像中的应用。
Langmuir. 2012 Mar 6;28(9):4464-71. doi: 10.1021/la204289k. Epub 2012 Feb 24.
8
Assessment of gold nanoparticles as a size-dependent vaccine carrier for enhancing the antibody response against synthetic foot-and-mouth disease virus peptide.评估金纳米颗粒作为一种尺寸依赖性疫苗载体，以增强针对合成口蹄疫病毒肽的抗体反应。
Nanotechnology. 2010 May 14;21(19):195101. doi: 10.1088/0957-4484/21/19/195101. Epub 2010 Apr 19.
9
Influence of gold nanoparticles of varying size in improving the lipase activity within cationic reverse micelles.不同粒径的金纳米粒子对阳离子反胶束中脂肪酶活性的影响。
Chemistry. 2010 Feb 8;16(6):1941-50. doi: 10.1002/chem.200901300.
10
The interaction between casein micelles and gold nanoparticles.酪蛋白胶束与金纳米颗粒之间的相互作用。
J Colloid Interface Sci. 2009 Apr 1;332(1):265-9. doi: 10.1016/j.jcis.2008.12.043. Epub 2008 Dec 24.

引用本文的文献

1
Latest Findings on the Effects of Gold Nanoparticles on the Storage Quality of Blood Products (2011-2022) - A Narrative Review.金纳米颗粒对血液制品储存质量影响的最新研究结果（2011 - 2022年）——一项叙述性综述
Curr Drug Deliv. 2025;22(5):537-551. doi: 10.2174/0115672018316266240909075316.
2
Anti-CD64 Antibody-Conjugated PLGA Nanoparticles Containing Methotrexate and Gold for Theranostics Application in Rheumatoid Arthritis.载甲氨蝶呤和金的抗 CD64 抗体偶联 PLGA 纳米粒用于类风湿关节炎的诊疗一体化应用。
AAPS PharmSciTech. 2024 Jan 24;25(1):22. doi: 10.1208/s12249-024-02733-w.
3
A Review on the Synthesis and Functionalization of Gold Nanoparticles as a Drug Delivery Vehicle.金纳米粒子作为药物传递载体的合成与功能化研究综述。
Int J Nanomedicine. 2020 Dec 7;15:9823-9857. doi: 10.2147/IJN.S279094. eCollection 2020.
4
Protein-Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles.蛋白质-纳米颗粒相互作用：纳米颗粒上的冠形成和蛋白质构象变化。
Int J Nanomedicine. 2020 Aug 6;15:5783-5802. doi: 10.2147/IJN.S254808. eCollection 2020.
5
Cytotoxicity and Apoptosis Inducing Activities of 2-Amino-4H-chromene-3-carbonitrile Derivatives Loaded on Gold Nanoparticles Against Human Breast Cancer Cell Line T47D.负载于金纳米颗粒上的2-氨基-4H-色烯-3-腈衍生物对人乳腺癌细胞系T47D的细胞毒性和诱导凋亡活性
Indian J Clin Biochem. 2015 Apr;30(2):140-9. doi: 10.1007/s12291-014-0417-5. Epub 2014 Jan 31.
6
In vitro antitumor evaluation of 4H-chromene-3-carbonitrile derivatives as a new series of apoptotic inducers.4H-色烯-3-腈衍生物作为新型凋亡诱导剂的体外抗肿瘤评价
Tumour Biol. 2014 Jun;35(6):5845-55. doi: 10.1007/s13277-014-1775-6. Epub 2014 Mar 19.

本文引用的文献

1
Structure and function of nanoparticle-protein conjugates.纳米颗粒-蛋白质缀合物的结构与功能。
Biomed Mater. 2008 Sep;3(3):034001. doi: 10.1088/1748-6041/3/3/034001. Epub 2008 Aug 8.
2
Gold nanoparticle-based surface enhanced Raman scattering spectroscopic assay for the detection of protein-protein interactions.基于金纳米颗粒的表面增强拉曼散射光谱分析法用于检测蛋白质-蛋白质相互作用。
Anal Sci. 2008 Jul;24(7):907-10. doi: 10.2116/analsci.24.907.
3
Synthesis and bioconjugation of gold nanoparticles as potential molecular probes for light-based imaging techniques.金纳米颗粒作为基于光的成像技术潜在分子探针的合成与生物共轭
Int J Biomed Imaging. 2007;2007:29817. doi: 10.1155/2007/29817.
4
Synthesis, stability, and cellular internalization of gold nanoparticles containing mixed peptide-poly(ethylene glycol) monolayers.含有混合肽-聚乙二醇单分子层的金纳米颗粒的合成、稳定性及细胞内化
Anal Chem. 2007 Mar 15;79(6):2221-9. doi: 10.1021/ac061578f. Epub 2007 Feb 9.
5
pH-dependent protein conformational changes in albumin:gold nanoparticle bioconjugates: a spectroscopic study.白蛋白中pH依赖的蛋白质构象变化：金纳米颗粒生物共轭物：一项光谱研究
Langmuir. 2007 Feb 27;23(5):2714-21. doi: 10.1021/la062064e. Epub 2007 Jan 24.
6
Rigid, specific, and discrete gold nanoparticle/antibody conjugates.刚性、特异性且离散的金纳米颗粒/抗体缀合物。
J Am Chem Soc. 2006 Mar 1;128(8):2635-40. doi: 10.1021/ja0555668.
7
Chemistry and properties of nanocrystals of different shapes.不同形状纳米晶体的化学性质与特性
Chem Rev. 2005 Apr;105(4):1025-102. doi: 10.1021/cr030063a.
8
Labeling ribonuclease S with a 3 nm Au nanoparticle by two-step assembly.
Nano Lett. 2005 Mar;5(3):519-22. doi: 10.1021/nl0479031.
9
Recent advances in the liquid-phase syntheses of inorganic nanoparticles.无机纳米粒子液相合成的最新进展。
Chem Rev. 2004 Sep;104(9):3893-946. doi: 10.1021/cr030027b.
10
Cellular trajectories of peptide-modified gold particle complexes: comparison of nuclear localization signals and peptide transduction domains.肽修饰的金颗粒复合物的细胞轨迹：核定位信号与肽转导结构域的比较
Bioconjug Chem. 2004 May-Jun;15(3):482-90. doi: 10.1021/bc034189q.

天冬氨酸包覆的金纳米颗粒的合成及其与FVIII蛋白和FVIII抗体的偶联

Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody.

作者信息

Zarabi Maryam Farahnak, Farhangi Ali, Mazdeh Samaneh Khademi, Ansarian Zahra, Zare Davod, Mehrabi Mohammad Reza, Akbarzadeh Azim

机构信息

Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran ; Zanjan University, Zanjan, Iran.

Pilot Biotechnology Department, Pasteur Institute of Iran, No. 358, 12 Farvardin Street, Jomhoori Avenue, 13169-43551 Tehran, Iran.

出版信息

Indian J Clin Biochem. 2014 Apr;29(2):154-60. doi: 10.1007/s12291-013-0323-2. Epub 2013 Apr 2.

DOI:10.1007/s12291-013-0323-2
PMID:24757296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3990797/
Abstract

Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV-Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP-protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.

摘要

羧酸盐修饰的金纳米颗粒（GNPs）通过天冬氨酸在水中还原氯金酸的简单一步法合成。通过紫外可见光谱、动态光散射（DLS）和透射电子显微镜对GNPs进行鉴定。蛋白质分子与功能化纳米颗粒的缀合通过静电相互作用进行。通过凝胶电泳对GNP-蛋白质缀合物进行表征。功能化GNPs与蛋白质分子之间的相互作用导致蛋白质与GNPs缀合后蛋白质结构的构象转变。通过荧光光谱和圆二色光谱对该过程进行了研究。