Rayavarapu Raja Gopal, Petersen Wilma, Ungureanu Constantin, Post Janine N, van Leeuwen Ton G, Manohar Srirang
Biophysical Engineering Group, Institute for Biomedical Technology (BMTI), Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
Int J Biomed Imaging. 2007;2007:29817. doi: 10.1155/2007/29817.
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the "optical imaging window." The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular probes in the field of biomedical imaging are also discussed.
我们已经合成并表征了在“光学成像窗口”内具有光消光带的金纳米颗粒（球体和棒体）。这些纳米颗粒强烈的等离子体共振驱动吸收和散射峰使其适合作为光学成像技术的造影剂。此外，我们已将这些金纳米颗粒与针对过表达HER2的SKBR3乳腺癌细胞的小鼠单克隆抗体偶联。生物偶联方案基于抗体与金表面的静电和疏水相互作用的组合使用非共价结合模式。我们讨论了合成和生物偶联方案的各个方面以及功能化纳米颗粒的表征结果。还讨论了这些潜在分子探针在生物医学成像领域的一些拟议应用。
