Dayal Meena, Sagar Shreshtha, Chaurasia Amrita, Singh Urvashi
Moti Lal Nehru Medical College, 347/1, Tagore Town, Allahabad, 211002 Uttar Pradesh India.
J Obstet Gynaecol India. 2014 Apr;64(2):130-3. doi: 10.1007/s13224-013-0482-3. Epub 2013 Dec 3.
The aim of this study was to determine day 3 Serum AMH, FSH, LH, Estradiol (E2), Inhibin B levels, ovarian volume, and antral follicular count to assess ovarian function.
This study was conducted on 130 infertile women between age 18 and 43 years. Day 3 Serum AMH level was estimated by sandwich enzyme immunoassay; Serum FSH, S. LH, S. E2, by solid-phase two-site chemiluminescent immunometric assay; Inhibin B by ELISA; and Ovarian volume and AFC, by transvaginal ultrasonography.
With advancing age, Serum AMH level (p < 0.0001), AFC (p < 0.05), ovarian volume (>0.05), and Inhibin B (>0.05) were decreased, and Serum FSH (p < 0.05), LH (p > 0.05), and E2 (p < 0.05) were increased. Serum AMH level was 4-6.8 ng/ml with optimal fertility in 26.15 % cases and 2.2-4.0 ng/ml with satisfactory fertility in 53.85 % cases. Serum AMH levels were more strongly correlated with AFC (p < 0.0001) and ovarian volume (p < 0.0001).
Serum AMH levels were more robustly correlated with AFC than FSH, LH, E2, and Inhibin B on day 3 of the cycle. This suggested that serum AMH might be taken as single test to reflect ovarian reserve.
本研究旨在测定第3天血清抗缪勒管激素（AMH）、卵泡刺激素（FSH）、黄体生成素（LH）、雌二醇（E2）、抑制素B水平、卵巢体积及窦卵泡计数，以评估卵巢功能。
本研究对130名年龄在18至43岁之间的不孕女性进行。采用夹心酶免疫测定法估计第3天血清AMH水平；采用固相双位点化学发光免疫分析法测定血清FSH、促黄体生成素（S. LH）、雌二醇（S. E2）；采用酶联免疫吸附测定法测定抑制素B；采用经阴道超声检查测定卵巢体积和窦卵泡计数。
随着年龄增长，血清AMH水平（p < 0.0001）、窦卵泡计数（p < 0.05）、卵巢体积（>0.05）及抑制素B（>0.05）降低，血清FSH（p < 0.05）、LH（p > 0.05）及E2（p < 0.05）升高。血清AMH水平在26.15%的病例中为4 - 6.8 ng/ml时生育力最佳，在53.85%的病例中为2.2 - 4.0 ng/ml时生育力良好。血清AMH水平与窦卵泡计数（p < 0.0001）和卵巢体积（p < 0.0001）的相关性更强。
在月经周期第3天，血清AMH水平与窦卵泡计数的相关性比FSH、LH、E2及抑制素B更强。这表明血清AMH可作为反映卵巢储备的单一检测指标。
