Nonomura A, Mizukami Y, Matsubara F, Izumi R, Nakanuma Y, Hayashi M, Watanabe K, Takayanagi N
Department of Surgery, Kanazawa University Hospital, Japan.
Liver. 1989 Aug;9(4):205-15. doi: 10.1111/j.1600-0676.1989.tb00401.x.
The immunohistochemical localization of human chorionic gonadotropin (HCG) and alpha-fetoprotein (AFP) was studied in 44 cases with cholangiocarcinoma (CC) to determine the correlation to the expression of ras oncogene product p21 on tumor cells. HCG-immunoreactivity was found in 10 of 44 cases (23%) and AFP in only one (2.3%), whereas the expression of ras p21 was demonstrated in 39 (88.6%). The incidence of HCG-positive cells within the tumor was less than 1% in 8 of 10. The incidence of AFP-positive cells was less than 0.01%. All were histologically classified as adenocarcinoma and none of them had histologic features of trophoblastic tumors, yolk sac tumor or hepatocellular carcinoma. Nine of 10 HCG-positive and one AFP-positive CC expressed ras p21 on their tumor cells. However, one HCG-positive CC was negative for ras p21, though the incidence of HCG-positive cells within the tumor was 25%. HCG- and AFP-immunoreactivity was more frequently observed in poorly or undifferentiated tumor cells than in moderately or well-differentiated areas, whereas the expression of ras p21 was more diffuse in well-differentiated tumor and stronger in moderately differentiated areas, but rarely found in poorly and undifferentiated tumor. These results suggest that HCG production by CC of the usual adenocarcinoma variety is not rare, when compared to AFP production, and is preferentially localized in a small number of poorly differentiated and undifferentiated carcinoma cells, and there is no correlation between the production of HCG or AFP and the expression of ras p21 in CCs.
对44例胆管癌（CC）患者进行了人绒毛膜促性腺激素（HCG）和甲胎蛋白（AFP）的免疫组化定位研究，以确定其与肿瘤细胞中ras癌基因产物p21表达的相关性。44例中有10例（23%）发现有HCG免疫反应性，仅1例（2.3%）有AFP免疫反应性，而ras p21表达见于39例（88.6%）。10例中有8例肿瘤内HCG阳性细胞发生率低于1%。AFP阳性细胞发生率低于0.01%。所有病例组织学均分类为腺癌，均无滋养层肿瘤、卵黄囊瘤或肝细胞癌的组织学特征。10例HCG阳性和1例AFP阳性的CC肿瘤细胞均表达ras p21。然而，1例HCG阳性的CC尽管肿瘤内HCG阳性细胞发生率为25%，但ras p21呈阴性。HCG和AFP免疫反应性在低分化或未分化肿瘤细胞中比在中分化或高分化区域更常见，而ras p21的表达在高分化肿瘤中更弥漫，在中分化区域更强，但在低分化和未分化肿瘤中很少见。这些结果表明，与AFP产生相比，普通腺癌类型的CC产生HCG并不罕见，且优先定位于少数低分化和未分化癌细胞中，并且CC中HCG或AFP的产生与ras p21的表达之间无相关性。
