Nonomura A, Ohta G, Hayashi M, Izumi R, Watanabe K, Takayanagi N, Matsubara F
Liver. 1987 Jun;7(3):142-8. doi: 10.1111/j.1600-0676.1987.tb00334.x.
An expression of ras p21 proteins on cholangiocarcinoma (CC) (intrahepatic bile duct carcinoma) cells was examined by an immunoperoxidase method using an appropriate dilution of mouse monoclonal antibody RAP-5, with which no positive staining was obtained in livers with normal histology. Of 44 CCs examined 39 were positive for the antigens; well-differentiated adenocarcinoma usually showed a diffuse weak, cytoplasmic staining in nearly all tumor cells with the same staining intensity, while in moderately and poorly differentiated adenocarcinoma the expression of p21 varied markedly in intensity from cell to cell in the same cell nest. The number of positive cells decreased with the grade of tumor, and no or little staining was observed in undifferentiated areas. These findings indicate that the expression of ras p21 antigens was lost with increasing dedifferentiation of tumor cells. Carcinoembryonic antigens (CEA) were positive in 42 of 44 CCS. Well-differentiated adenocarcinoma expressed CEA along the apical surfaces of the tumor glands. With the dedifferentiation of tumor cells, the expression of CEA became prominent not only at the apical surfaces but also on the basolateral surfaces and in the cytoplasms, and further in the surrounding stromal tissue. There was no clear-cut correlation between the expression of p21 antigens and the production of CEA in CCs.
采用免疫过氧化物酶法，使用适当稀释的小鼠单克隆抗体RAP - 5检测胆管癌（CC，肝内胆管癌）细胞中ras p21蛋白的表达情况，在组织学正常的肝脏中未获得阳性染色。在所检测的44例CC中，39例抗原呈阳性；高分化腺癌通常在几乎所有肿瘤细胞中显示弥漫性弱阳性的胞质染色，且染色强度相同，而在中分化和低分化腺癌中，p21在同一细胞巢内的细胞间表达强度差异明显。阳性细胞数量随肿瘤分级降低，在未分化区域未观察到染色或仅有少量染色。这些发现表明，随着肿瘤细胞去分化程度增加，ras p21抗原的表达丧失。癌胚抗原（CEA）在44例CC中的42例呈阳性。高分化腺癌在肿瘤腺管的顶端表面表达CEA。随着肿瘤细胞的去分化，CEA的表达不仅在顶端表面变得突出，而且在基底外侧表面和细胞质中以及周围基质组织中也变得突出。在CC中，p21抗原的表达与CEA的产生之间没有明确的相关性。
