猪胸膜肺炎放线杆菌（溶血嗜血杆菌）中一种麦芽糖诱导型主要外膜蛋白的鉴定。

Identification of a maltose-inducible major outer membrane protein in Actinobacillus (Haemophilus) pleuropneumoniae.

作者信息

Deneer H G, Potter A A

机构信息

Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.

出版信息

Microb Pathog. 1989 Jun;6(6):425-32. doi: 10.1016/0882-4010(89)90084-3.

DOI:10.1016/0882-4010(89)90084-3

PMID:2475740

Abstract

The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropneumoniae expressing the 42 kDa OMP were unable to bind lambda phage. Similar proteins were also induced in A. pleuropneumoniae isolates representing serotypes 2 to 7 with the exception of serotype 4; however, not all isolates of any given serotype expressed a maltose-inducible OMP. Western immunoblotting using convalescent antiserae against the serotype 1 A. pleuropneumoniae indicated that the 42 kDa OMP was expressed in vivo and was cross-reactive with the maltose-inducible OMPs from other serotypes.

摘要

在胸膜肺炎放线杆菌（血清型1）的生长培养基中添加麦芽糖会诱导产生一种分子量为42 kDa的外膜蛋白（OMP）。这种蛋白具有孔蛋白样特性，因为它与肽聚糖相关，并且对胰蛋白酶的蛋白水解具有抗性。表达42 kDa OMP的胸膜肺炎放线杆菌无法结合λ噬菌体。除血清型4外，在代表血清型2至7的胸膜肺炎放线杆菌分离株中也诱导出了类似的蛋白；然而，任何给定血清型的并非所有分离株都表达麦芽糖诱导型OMP。使用针对血清型1胸膜肺炎放线杆菌的恢复期抗血清进行的Western免疫印迹表明，42 kDa OMP在体内表达，并且与来自其他血清型的麦芽糖诱导型OMP具有交叉反应性。