MacInnes J I, Rosendal S
Infect Immun. 1987 Jul;55(7):1626-34. doi: 10.1128/iai.55.7.1626-1634.1987.
Outer membrane protein (OMP)-enriched extracts and whole-cell protein preparations of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. Both the OMP-enriched and whole-cell protein profiles of Actinobacillus suis, A. pleuropneumoniae (NAD-independent biovar), A. lignieresii, and Pasteurella haemolytica were very similar to those of H. pleuropneumoniae serotypes 1 to 8. Antisera prepared against H. pleuropneumoniae typically recognized three major OMP antigens with approximate molecular weights of 17,000 (17K), 32K, and 42K in immunoblots of H. pleuropneumoniae serotypes 1 to 8, Actinobacillus spp., and P. haemolytica. Antisera prepared against Actinobacillus spp. and Haemophilus sp. "minor group" also recognized these 17K, 32K, and 42K antigens. Using absorbed sera, we demonstrated that the 17K antigen had an epitope (or epitopes) common to all the gram-negative organisms examined, including Escherichia coli. The 32K and 42K antigens had epitopes common to members of the family Pasteurellaceae but, in the case of the 32K antigen, also contained unique epitopes. These results provide a basis for understanding the lack of specificity of serodiagnostic tests for H. pleuropneumoniae infection and provide another line of evidence for the association of H. pleuropneumoniae with the genus Actinobacillus.
采用聚丙烯酰胺凝胶电泳和免疫印迹法，对胸膜肺炎放线杆菌（嗜血气杆菌）及相关微生物的外膜蛋白（OMP）富集提取物和全细胞蛋白制剂进行了检测。猪放线杆菌、胸膜肺炎放线杆菌（不依赖烟酰胺腺嘌呤二核苷酸生物变种）、里氏放线杆菌和溶血巴斯德菌的OMP富集蛋白谱和全细胞蛋白谱与胸膜肺炎嗜血气杆菌1至8型的蛋白谱非常相似。针对胸膜肺炎嗜血气杆菌制备的抗血清，在胸膜肺炎嗜血气杆菌1至8型、放线杆菌属和溶血巴斯德菌的免疫印迹中，通常能识别出三种主要的OMP抗原，其近似分子量分别为17000（17K）、32K和42K。针对放线杆菌属和嗜血气杆菌“小群”制备的抗血清也能识别这些17K、32K和42K抗原。利用吸收后的血清，我们证明17K抗原具有所有被检测革兰氏阴性菌共有的一个或多个表位，包括大肠杆菌。32K和42K抗原具有巴斯德菌科成员共有的表位，但就32K抗原而言，也含有独特的表位。这些结果为理解胸膜肺炎嗜血气杆菌感染血清学诊断试验缺乏特异性提供了依据，并为胸膜肺炎嗜血气杆菌与放线杆菌属的关联提供了另一系列证据。
