问号钩端螺旋体和双曲钩端螺旋体中大核糖体RNA基因的数量。

The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa.

作者信息

Fukunaga M, Mifuchi I

机构信息

Faculty of Pharmacy and Pharmaceutical Science, University of Fukuyama, Hiroshima.

出版信息

Microbiol Immunol. 1989;33(6):459-66. doi: 10.1111/j.1348-0421.1989.tb01995.x.

DOI:10.1111/j.1348-0421.1989.tb01995.x

PMID:2475749

Abstract

We determined the number of large ribosomal RNA genes in five strains of Leptospira by hybridization of 15 restriction endonuclease digests of genomic DNA to the [32P]-labeled fragment of 23s rRNA gene. Almost all the restriction gels gave two radioactive bands. The conclusion from these results is that there are at least two rRNA genes in these leptospiral strains. Furthermore, the hybridization patterns of L. icterohaemorrhagiae strains Ictero No. I and RGA are almost identical. The number of rRNA genes and taxonomic relationships of these leptospires were discussed.

摘要

我们通过将基因组DNA的15种限制性内切酶消化产物与[32P]标记的23s rRNA基因片段杂交，确定了五株钩端螺旋体中大核糖体RNA基因的数量。几乎所有的限制性凝胶都出现了两条放射性条带。这些结果得出的结论是，这些钩端螺旋体菌株中至少有两个rRNA基因。此外，出血性黄疸钩端螺旋体菌株Ictero No. I和RGA的杂交模式几乎相同。本文还讨论了这些钩端螺旋体的rRNA基因数量和分类关系。