Subbarao M N, Apirion D
Department of Microbiology and Immunology, Washington University Medical School, St. Louis, MO 63110.
Mol Gen Genet. 1989 Jun;217(2-3):499-504. doi: 10.1007/BF02464923.
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated. The T1 fingerprint of 10Sa RNA and the larger molecule is very similar, but the latter contains additional oligonucleotides. We show that the larger RNA is converted to the smaller, mature RNA. The precursor molecule starts with an adenosine triphosphate and is therefore a primary transcript. RNase E is not the enzyme that processes p10Sa (precursor 10Sa) RNA into 10Sa RNA. The cell extract contains an activity that carries out this conversion. This activity requires the dication Mn2+.
携带编码10Sa RNA质粒的菌株在RNA加工酶核糖核酸酶E失活时会合成一种更大的分子。10Sa RNA和更大分子的T1指纹图谱非常相似，但后者包含额外的寡核苷酸。我们发现更大的RNA会转化为更小的成熟RNA。前体分子以三磷酸腺苷开始，因此是初级转录本。核糖核酸酶E不是将p10Sa（前体10Sa）RNA加工成10Sa RNA的酶。细胞提取物中含有进行这种转化的活性。这种活性需要二价阳离子锰离子。
