Pulido-Landínez Martha, Washington Paul, Thornton Jay Kay, Zhang Yi, Sánchez-Ingunza Roxana, Banda Alejandro, Guard Jean, Nascimento Vladimir P, Magee Danny L, Mauel Michael J
Avian Dis. 2014 Mar;58(1):64-70. doi: 10.1637/10636-081213-Reg.1.
To obtain information about Salmonella from commercial birds and poultry environments within Mississippi, 50 Salmonella enterica isolates were collected and characterized by intergenic sequence ribotyping (ISR) serotyping and by determining antimicrobial resistance. ISR assigned serotype to all 50 Salmonella enterica isolates whereas the Kauffman-White-LeMinor antibody-based scheme assigned serotype to 48. Agreement between both methods was K = 89.58. Within the set, 12 serotypes were detected. The antimicrobial resistance patterns (ARP) of 12 serotypes, namely Enteritidis, Typhimurium, Kentucky, Bredeney, Mbandaka, Saintpaul, Montevideo, Cubana, Lille, Senftenberg, Johannesburg, and one serotype UN0094, were determined using minimum inhibitory concentration values. The antibiograms demonstrated differences between Salmonella serotypes and among isolates of the same serotype. All isolates were 100% susceptible to enrofloxacin and trimethoprim/sulfamethoxazole. The number of antimicrobials to which the isolates were resistant ranged from two to nine. Twenty-two different ARPs were identified and ARP1, with resistance to spectinomycin and sulfadimethoxine, was most frequently observed. Forty isolates (80%) were resistant to three or more antimicrobials and were thus designated multidrug resistant. Detection of a unique serotype, and variation in antibiograms within the set, demonstrates that it is important to survey isolates periodically from a region to follow epidemiologic trends.
为获取密西西比州商业禽类及家禽养殖环境中沙门氏菌的相关信息，收集了50株肠炎沙门氏菌分离株，并通过基因间序列核糖分型（ISR）、血清分型以及测定抗菌药物耐药性对其进行特征分析。ISR可对所有50株肠炎沙门氏菌分离株进行血清分型，而基于考夫曼-怀特-莱Minor抗体的方法可对48株进行血清分型。两种方法的一致性系数K = 89.58。在这一组分离株中，检测到了12种血清型。使用最低抑菌浓度值测定了12种血清型的抗菌药物耐药模式（ARP），这12种血清型分别为肠炎型、鼠伤寒型、肯塔基型、布雷登尼型、姆班达卡型、圣保罗型、蒙得维的亚型、古巴型、里尔型、森夫滕贝格型、约翰内斯堡型以及一种血清型UN0094。抗菌谱显示不同沙门氏菌血清型之间以及同一血清型的分离株之间存在差异。所有分离株对恩诺沙星和甲氧苄啶/磺胺甲恶唑均100%敏感。分离株耐药的抗菌药物数量从2种到9种不等。共鉴定出22种不同的ARP，其中对壮观霉素和磺胺二甲氧嘧啶耐药的ARP1最为常见。40株（80%）分离株对三种或更多抗菌药物耐药，因此被认定为多重耐药。独特血清型的检测以及该组分离株抗菌谱的差异表明，定期对某一地区的分离株进行监测以跟踪流行病学趋势非常重要。
