University of Kentucky, Department of Chemistry, Lexington, KY 40506, United States.
University of Kentucky, Department of Biosystems & Agricultural Engineering, Lexington, KY 40546, United States.
Bioresour Technol. 2014 Jun;162:273-8. doi: 10.1016/j.biortech.2014.03.151. Epub 2014 Apr 5.
In an attempt to mimic white-rot fungi lignin degradation via in vivo Fenton chemistry, solution phase Fenton chemistry (10 g biomass, 176 mmol hydrogen peroxide and 1.25 mmol Fe(2+) in 200 mL of water) was applied to four different biomass feedstocks. An enzymatic saccharification of Fenton pretreated biomass showed an average 212% increase relative to untreated control across all four feedstocks (P<0.05, statistically significant). A microbial fermentation of the same Fenton pretreated biomass showed a threefold increase in gas production upon a sequential co-culture with Clostridium thermocellum and Clostridium beijerinckii. These results demonstrate the use of solution phase Fenton chemistry as a viable pretreatment method to make cellulose more bioavailable for microbial biofuel conversion.
为了模拟白腐真菌木质素降解的体内芬顿化学，我们采用溶液相芬顿化学法（10g 生物质、176mmol 过氧化氢和 1.25mmolFe(2+)在 200mL 水中）处理了四种不同的生物质原料。与未经处理的对照组相比，经芬顿预处理的生物质的酶解糖化显示出平均 212%的提高（P<0.05，具有统计学意义）。对同一批经芬顿预处理的生物质进行微生物发酵，在与嗜热梭菌和拜氏梭菌进行顺序共培养后，气体产量增加了三倍。这些结果表明，溶液相芬顿化学法是一种可行的预处理方法，可以使纤维素更有利于微生物生物燃料转化。
