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白色念珠菌基因操作的分子遗传学技术

Molecular genetic techniques for gene manipulation in Candida albicans.

作者信息

Xu Qiu-Rong, Yan Lan, Lv Quan-Zhen, Zhou Mi, Sui Xue, Cao Yong-Bing, Jiang Yuan-Ying

机构信息

Department of Traditional Chinese Medicine; College of Pharmacy; Fujian University of Traditional Chinese Medicine; Fuzhou, Fujian PR China.

Center for New Drug Research; School of Pharmacy; Second Military Medical University; Shanghai, PR China.

出版信息

Virulence. 2014 May 15;5(4):507-20. doi: 10.4161/viru.28893. Epub 2014 Apr 23.

DOI:10.4161/viru.28893
PMID:24759671
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4063812/
Abstract

Candida albicans is one of the most common fungal pathogen in humans due to its high frequency as an opportunistic and pathogenic fungus causing superficial as well as invasive infections in immunocompromised patients. An understanding of gene function in C. albicans is necessary to study the molecular basis of its pathogenesis, virulence and drug resistance. Several manipulation techniques have been used for investigation of gene function in C. albicans, including gene disruption, controlled gene expression, protein tagging, gene reintegration, and overexpression. In this review, the main cassettes containing selectable markers used for gene manipulation in C. albicans are summarized; the advantages and limitations of these cassettes are discussed concerning the influences on the target gene expression and the virulence of the mutant strains.

摘要

白色念珠菌是人类最常见的真菌病原体之一，因为它作为一种机会性致病真菌，在免疫功能低下的患者中引起浅表和侵袭性感染的频率很高。了解白色念珠菌的基因功能对于研究其发病机制、毒力和耐药性的分子基础是必要的。已经使用了几种操作技术来研究白色念珠菌的基因功能，包括基因破坏、可控基因表达、蛋白质标记、基因重新整合和过表达。在这篇综述中，总结了用于白色念珠菌基因操作的含有选择标记的主要盒式结构；讨论了这些盒式结构在对靶基因表达和突变菌株毒力的影响方面的优点和局限性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/e2745ecdda71/viru-5-507-g8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/e2745ecdda71/viru-5-507-g8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/351407ab4ed9/viru-5-507-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/824080174535/viru-5-507-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/0930df5fb60d/viru-5-507-g3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42c4/4063812/4ebf4f56b8ec/viru-5-507-g5.jpg
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