De Brabander M, Geerts H, Nuydens R, Nuyens R
Department of Life Sciences, Janssen Research Foundation, Beerse, Belgium.
Am J Anat. 1989 Jun-Jul;185(2-3):282-95. doi: 10.1002/aja.1001850221.
This contribution describes a new microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter as dynamic markers at the light microscopic level. The technique, called nanovid microscopy, is based on the use of contrast enhancement by video techniques and digital image processing. Two applications on living cells are discussed. The first is the receptor-mediated endocytosis of the transferrin receptor, which for the first time can be followed in real time. A second application documents the motion of cell-surface glycoproteins on PTK2-cells. In addition, nanovid microscopy allows the number of gold particles in immunogold staining to be quantified easily. With this tool, the translocation of specific molecules in living cells becomes one of the most enjoyable activities to observe.
本论文介绍了一种新的显微技术，旨在将直径为20 - 40纳米的胶体金颗粒作为动态标记物，在光学显微镜水平上进行可视化观察。该技术称为纳米视频显微镜，它基于视频技术和数字图像处理来增强对比度。文中讨论了该技术在活细胞上的两个应用。第一个应用是转铁蛋白受体的受体介导内吞作用，这是首次能够实时追踪该过程。第二个应用记录了PTK2细胞表面糖蛋白的运动。此外，纳米视频显微镜还能轻松定量免疫金染色中胶体金颗粒的数量。借助这个工具，观察活细胞中特定分子的转运成为了最有趣的活动之一。
