De Brabander M, Geuens G, Nuydens R, Moeremans M, De Mey J
Cytobios. 1985;43(174S):273-83.
Colloidal gold particles of 20 to 40 nm diameter stabilized with polyethylene glycol (PEG) were microinjected in PTK2 cells. Aggregates and individual particles, which are smaller than the theoretical limit of resolution of the optical microscope and invisible to the eye are discernible from organelles by reflection of polarized light. They are optimally visualized using transmitted light and electronic subtraction of diffuse background light. The gold particles show saltatory motion. The direction, speed, median distance travelled and frequency of saltations are indiscernible from measurements made on cell organelles in the same preparations. Because microtubule treadmilling has been implicated as a potential motor for organelle motility, gold particles coupled to monoclonal antibodies, recognizing the alpha-subunit of tubulin (Kilmartin et al., 1982), were injected. These particles, often forming linear arrays, assumed entirely fixed positions in the cell. The results suggest that there is a transport system associated with microtubules which can carry synthetic particles through the cell without the need for them being covered with specific proteins. Microtubule treadmilling does not seem to be involved. The possibility of following 20-40 nm particles and probably even smaller ones, that can be coupled to most proteins, within living cells provides a tool of wide applicability to study the fate and behaviour of such proteins. It is suggested that this new method be called nanoparticle video ultramicroscopy or nanovid ultramicroscopy.
将用聚乙二醇（PEG）稳定的直径为20至40纳米的胶体金颗粒显微注射到PTK2细胞中。通过偏振光反射，可从细胞器中分辨出比光学显微镜理论分辨率极限小且肉眼不可见的聚集体和单个颗粒。使用透射光和对漫射背景光进行电子减法可实现最佳可视化。金颗粒呈现跳跃运动。从对同一制剂中细胞器的测量结果来看，跳跃的方向、速度、行进的中位距离和频率无法辨别。由于微管踏车运动被认为是细胞器运动的潜在动力，因此注射了与识别微管蛋白α亚基的单克隆抗体偶联的金颗粒（Kilmartin等人，1982年）。这些颗粒通常形成线性阵列，在细胞中占据完全固定的位置。结果表明，存在一种与微管相关的运输系统，它可以携带合成颗粒穿过细胞，而无需颗粒被特定蛋白质覆盖。微管踏车运动似乎未参与其中。在活细胞内追踪可与大多数蛋白质偶联的20 - 40纳米颗粒甚至可能更小颗粒的可能性，为研究此类蛋白质的命运和行为提供了一种广泛适用的工具。建议将这种新方法称为纳米颗粒视频超显微术或纳米视频超显微术。
