Lamb J R, Bal V, Rothbard J B, Mehlert A, Mendez-Samperio P, Young D B
MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
J Autoimmun. 1989 Jun;2 Suppl:93-100. doi: 10.1016/0896-8411(89)90120-0.
The 65 kD protein of mycobacteria is an immunodominant antigen for both T and B lymphocytes. Sequence analysis has revealed that this protein belongs to the highly conserved family of stress proteins, related to the GroEL gene product of E. coli, that are present in all cells from bacteria to man. We demonstrate here that human T cells from healthy individuals and disease sites are able to recognize determinants within the 65 kD protein that are either specific for M. tuberculosis or are conserved between GroEL of mycobacterial, E. coli or human origin. The induction of T cells that recognize with cross-reactive sequences of GroEL may provide an explanation for the autoimmune phenomena often associated with infection by microbial pathogens. However, both the magnitude and the biological significance of this component of the T-cell repertoire reactive with self stress proteins will be influenced by local environmental factors as well as the MHC haplotype of the individual.
分枝杆菌的65kD蛋白是T淋巴细胞和B淋巴细胞的免疫显性抗原。序列分析表明，该蛋白属于应激蛋白的高度保守家族，与大肠杆菌的GroEL基因产物相关，存在于从细菌到人类的所有细胞中。我们在此证明，来自健康个体和疾病部位的人类T细胞能够识别65kD蛋白内对结核分枝杆菌特异或在分枝杆菌、大肠杆菌或人类来源的GroEL之间保守的决定簇。识别具有GroEL交叉反应序列的T细胞的诱导可能为通常与微生物病原体感染相关的自身免疫现象提供一种解释。然而，与自身应激蛋白反应的T细胞库这一组成部分的强度和生物学意义将受到局部环境因素以及个体的MHC单倍型的影响。
