Liang Xue-Juan, Zhang Shui-Han, Zhang Ping, Peng Fei, Ke Jian, Mi Ya-Nan
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):204-8.
A total of 152 strains of endophytic fungi were isolated from the barks of Eucommia ulmoides in three regions (Lueyang country, Zunyi country, Cili country). Based on morphological characteristics and analysis of ITS sequences, these strains were identified into 8 genera. Thereinto Phomopsis, Diaporthe and Alternaria were common genera to Eucommia barks from different sites. But the dominant genus was different: Alternaria was the dominant genus in the barks from Cili country, and Phomopsis was the dominant genus from Zunyi country, then Diaporthe was the one from Lueyang country. According to the similarity coefficient, the composition of the endophytic fungi was distinctly different between the barks from three sites. The diversity and species richness in Lueyang country and Cili country were found higher than those in Zunyi country. The evenness of endophytic fungi was 0.936 5 in Lueyang county, which was higher than 0.737 1 or 0.641 0 in Cili county or Zunyi county, respectively. After phylogenic analysis and calculating the genetic distances of typical strains belong to Phomopsis and its perfect stage--Diaporthe, there was very high genetic diversity in the two genera from our study. In conclusion, the community structure and diversity of endophytic fungi were significant different in Eucommia barks from the three habitats.
从三个地区（略阳县、遵义县、慈利县）的杜仲树皮中总共分离出152株内生真菌。基于形态特征和ITS序列分析，这些菌株被鉴定为8个属。其中，拟茎点霉属、间座壳属和链格孢属是不同产地杜仲树皮中的常见属。但优势属不同：链格孢属是慈利县树皮中的优势属，拟茎点霉属是遵义县树皮中的优势属，间座壳属是略阳县树皮中的优势属。根据相似系数，三个产地树皮中内生真菌的组成明显不同。发现略阳县和慈利县内生真菌的多样性和物种丰富度高于遵义县。略阳县内生真菌的均匀度为0.936 5，分别高于慈利县的0.737 1或遵义县的0.641 0。在对拟茎点霉及其有性阶段——间座壳属的典型菌株进行系统发育分析并计算遗传距离后，本研究中这两个属具有非常高的遗传多样性。总之，三个生境杜仲树皮中内生真菌的群落结构和多样性存在显著差异。
