Jia Dongsheng, Jia Xiaobin, Xue Jing, Shi Feng, Sun E, Chen Lingling, Zhang Zhenhai
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(7):857-60. doi: 10.4268/cjcmm20100711.
To optimize the conditions for enzymolysis of Icariin by snail hydrolase.
Take conversion rate as index, the effects of pH, temperature, reaction time, dosage of enzyme, concentration of Icariin and metal ion on hydrolysis were studied by single-factor designs and a L9 (3(4)) orthogonal design. The product was characterized through MS, 1H-NMR and 13C-NMR methods.
The optimum enzymolysis conversion rate was achieved at 37 degrees C, pH 6.0 acetic acid-sodium acetate buffer solution and 48 h. The Ca2+, Mg2+ and Zn2+ had activation on helicase slightly but the Fe2+ inhibited enzymolysis significantly. The production was presumed as icaritin on the basis of spectral evidences.
Snail hydrolase can be used for preparing of icaritin. The condition is mildness and suitable for industrialization.
