Laboratory of Microbiology and Immunology, School of Chinese and Western Integrative Medicine, Anhui University of Chinese Medicine, Hefei, 230038, China.
World J Microbiol Biotechnol. 2013 Dec;29(12):2373-8. doi: 10.1007/s11274-013-1405-2. Epub 2013 Jun 23.
Pseudomonas aeruginosa is a well-known clinical pathogen for its recalcitrant infection caused by biofilm formation which are initiated by flagella-mediated attachment. Sodium houttuyfonate (SH) is a natural phytoanticipin derivative of houttuynin and has anti-pathogenic effect on P. aeruginosa biofilm formation. In this paper, when using 1/2 × MIC SH, the diameter of P. aeruginosa swimming motility was sharply shortened to 36 % in 24 h incubation, and the fold changes of fliC required for swimming motility was 0.36 in 24 h cultivation, the adherence inhibition accounted for about 46 %, and the pyocyanin production decreased to 47 % after 1-day treatment and 56 % after 3-day treatment with obvious visual changes from dark green to light green, compared with the negative control. With the help of mass spectra and scanning electronic microscope, 1/2 × MIC SH was further testified to be enough to eradicate flagella and inhibit pyocyanin secretion of P. aeruginosa. The results do not only re-affirm the close interplay of attachment and virulence (i.e. swimming motility and pyocyanin), but also unravel the potential mechanism of SH on anti-biofilm of P. aeruginosa.
铜绿假单胞菌是一种众所周知的临床病原体，其顽强的感染是由生物膜形成引起的，生物膜形成是由鞭毛介导的附着引发的。虎耳草酸钠（SH）是鱼腥草素的天然植物抗毒素衍生物，对铜绿假单胞菌生物膜形成具有抗病原作用。在本文中，当使用 1/2×MIC SH 时，铜绿假单胞菌游动性的泳动直径在 24 小时孵育时急剧缩短至 36%，并且在 24 小时培养中，用于游动性的 fliC 的折叠变化为 0.36，粘附抑制约占 46%，在 1 天处理后，绿脓菌素的产量下降到 47%，在 3 天处理后下降到 56%，与阴性对照相比，颜色从深绿色变为浅绿色，有明显的视觉变化。借助质谱和扫描电子显微镜，进一步证明 1/2×MIC SH 足以消除鞭毛并抑制铜绿假单胞菌的绿脓菌素分泌。结果不仅再次证实了附着和毒力（即游动性和绿脓菌素）之间的紧密相互作用，还揭示了 SH 对铜绿假单胞菌抗生物膜的潜在机制。
