Gao Jia-Rong, Ji Wen-Bo, Jiang Hui, Chen Jin-Feng
Zhong Yao Cai. 2013 Oct;36(10):1635-9.
To observe the effects of extract from Ziziphus Spinosa Semen and Schisandrae Chinensis Fructus on the content of amino acid neurotransmitter in the hypothalamus of insomnia rats induced by P-Chlorophenylalanine (PCPA) and its mechanism.
The model of insomnia rats were established by PCPA intraperitoneal injection, after the modeling, all the therapeutic group were treated with corresponding drug for one week. The hypothalamus pathological changes of the rats were observed. The contents of GABA, Glu in the hypothalamus were detected by Elisa. The GABA, Glu protein expression were detected by immunohistochemical. GABA(A), R(alpha1) and GABA(A)R(gamma2) mRNA expressions were detected by RT-PCR.
Compared with model group, the content of GABA in the hypothalamus of rats increased obviously in the alcohol-water group (P < 0.05 or P < 0.01), while the content of Glu decreased obviously (P < 0.05 or P < 0.01).
The extract from Ziziphus Spinosae Semen and Schisandrae Chinensis Fructus has obviously Sedative-hypnotic effect. Its mechanism may be related to regulating the content of amino acid neurotransmitter in the hypothalamus of rats.
观察酸枣仁与五味子提取物对对氯苯丙氨酸（PCPA）致失眠大鼠下丘脑氨基酸类神经递质含量的影响及其作用机制。
采用腹腔注射PCPA建立失眠大鼠模型，造模后各治疗组给予相应药物治疗1周。观察大鼠下丘脑病理变化。采用酶联免疫吸附测定法检测下丘脑γ-氨基丁酸（GABA）、谷氨酸（Glu）含量。采用免疫组织化学法检测GABA、Glu蛋白表达。采用逆转录聚合酶链反应（RT-PCR）检测GABA(A)R(α1)和GABA(A)R(γ2)mRNA表达。
与模型组比较，醇水组大鼠下丘脑GABA含量明显升高（P<0.05或P<0.01），而Glu含量明显降低（P<0.05或P<0.01）。
酸枣仁与五味子提取物有明显的镇静催眠作用。其机制可能与调节大鼠下丘脑氨基酸类神经递质含量有关。
