Rong Chun-Lei, Dai Yong-Xia, Cui Ying
Henan College of Traditional Chinese Medicne, Zhengzhou 450008, China.
Zhong Yao Cai. 2008 Nov;31(11):1703-5.
To observe the effects of Semen Ziziphi Spinosae on the anxiety behavior of the yin deficiency mice, to filter the active material of Semen Ziziphi Spinosae and explore its anti-anxiety mechanism.
The yin deficiency mice model with intragastrical 320 mg/kg thyroid tablet for nine days exception normal control group. To observe yin deficiency model mice anxiety action by means of the elevated plus-maze and light-dark transitions of mice. After the experiment, peeled off cerebra at once. The left saved in liquid nitrogen for assaying GABA and Glu with TLCS, and right saved in formalin for the protein expression of GABAaR1 and NMDAR1 with immunohistochemical method.
(1) Compared with yin deficiency model group, Tabellae Diazepami group and the middle alcohol extracts group increased instinctively in the percentage of time and degree entering open-arm in eleveated plus maze, and the passing times in light-dark transitions increased instinctively (P<0.05). (2) Compared with yin deficiency model group, the contents of GABA increased evidently (P<0.05) and Glu reduced evidently (P<0.05) in Tabellae Diazepami group and the middle alcohol extracts group. (3) Compared with yin deficiency model group,the expression of GABAAR1 increased evidently (P<0.05) and NMDAR1 reduced evidently (P<0.05) in the diszepam group and the middle alcohol extracts group.
The alcohol extracts of Semen Ziziphi Spinosae have the anti-anxiety effects instinctively. Its mechanism may be related to increasing the GABA and expression of GABAAR1 and reducing the Glu and expression of NMDAR1.
观察酸枣仁对阴虚小鼠焦虑行为的影响，筛选酸枣仁的活性物质并探讨其抗焦虑机制。
除正常对照组外，采用灌胃320mg/kg甲状腺片9天的方法建立阴虚小鼠模型。通过高架十字迷宫和明暗箱转换实验观察阴虚模型小鼠的焦虑行为。实验结束后，立即断头取脑。左侧脑组织保存于液氮中，采用薄层扫描法测定γ-氨基丁酸（GABA）和谷氨酸（Glu）含量；右侧脑组织保存于甲醛中，采用免疫组织化学方法检测GABAaR1和NMDAR1蛋白表达。
（1）与阴虚模型组相比，地西泮组和酸枣仁醇提物中剂量组小鼠在高架十字迷宫实验中进入开放臂的时间百分比和程度显著增加，在明暗箱转换实验中穿梭次数显著增加（P<0.05）。（2）与阴虚模型组相比，地西泮组和酸枣仁醇提物中剂量组小鼠脑组织中GABA含量显著升高（P<0.05），Glu含量显著降低（P<0.05）。（3）与阴虚模型组相比，地西泮组和酸枣仁醇提物中剂量组小鼠脑组织中GABAAR1表达显著增加（P<0.05），NMDAR1表达显著降低（P<0.05）。
酸枣仁醇提物具有明显的抗焦虑作用，其机制可能与增加GABA含量及GABAAR1表达、降低Glu含量及NMDAR1表达有关。
