Fang Kun-Peng, Zhang Jian-Lin, Ren Yan-Hong, Qian Ye-Ben
First Affiliated Hospital of Anhui Medical University, Hefei, China E-mail :
Asian Pac J Cancer Prev. 2014;15(6):2655-61. doi: 10.7314/apjcp.2014.15.6.2655.
Talin-1 is a cytoskeleton protein that participates in cell migration and plays a role in tumor formation, migration, and metastasis in different types of cancer. Chinese investigators have observed that the levels of Talin-1 protein and mRNA expression in HCC tissues are significantly lower than in the adjacent non-cancerous tissue. However, Japanese investigators have reported that Talin-1 is upregulated in HCC. Tln2 as homologous gene of Tln-1, which encodes a very similar protein, but the role of Talin-2 is very little known in primary liver cancer (PLC). We investigated whether the expression of Talin-1 in PLC may be associated with the histological subtype as well as the role of Talin-1 in tumor cell invasion and migration using human hepatocellular carcinoma cell lines.
We measured the mRNA expression levels of Talin-1 and Talin-2 in five human liver cancer cell lines and normal human liver cell (LO2 cell line) by real-time PCR and the protein expression levels of Talin-1 by Western blot. Migration and invasion of the cells were assessed using transwell assays and cell scratch experiments, respectively, and proliferation was assessed by soft AGAR colony formation.
Talin-1 and Talin-2 expression differed significantly between the five human liver cancer cell lines and LO2 cell line (p<0.05). Compared with the LO2 cell line, the invasion and migration capabilities of the five cancer cell lines differed significantly (p<0.05). Similarly, the colony-forming ability differed (p<0.05).
High levels of Talin-1 expression are correlated with reduced invasion and migration as well as decreased malignancy in human liver cancer cell lines; the suppression of Talin-1 promotes invasion and migration. In addition, Talin-2 may be correlated with invasion and migration in human hepatocellular carcinoma.
踝蛋白-1是一种细胞骨架蛋白，参与细胞迁移，并在不同类型癌症的肿瘤形成、迁移和转移中发挥作用。中国研究人员观察到，肝癌组织中踝蛋白-1蛋白水平和mRNA表达显著低于相邻的非癌组织。然而，日本研究人员报告称，肝癌中踝蛋白-1上调。Tln2作为Tln-1的同源基因，编码一种非常相似的蛋白质，但Talin-2在原发性肝癌（PLC）中的作用鲜为人知。我们使用人肝癌细胞系研究了PLC中Talin-1的表达是否可能与组织学亚型相关，以及Talin-1在肿瘤细胞侵袭和迁移中的作用。
我们通过实时PCR测量了五种人肝癌细胞系和正常人肝细胞（LO2细胞系）中Talin-1和Talin-2的mRNA表达水平，并通过蛋白质印迹法测量了Talin-1的蛋白质表达水平。分别使用Transwell实验和细胞划痕实验评估细胞的迁移和侵袭能力，并通过软琼脂集落形成评估细胞增殖。
五种人肝癌细胞系和LO2细胞系之间Talin-1和Talin-2的表达存在显著差异（p<0.05）。与LO2细胞系相比，五种癌细胞系的侵袭和迁移能力存在显著差异（p<0.05）。同样，集落形成能力也存在差异（p<0.05）。
人肝癌细胞系中高水平的Talin-1表达与侵袭和迁移减少以及恶性程度降低相关；抑制Talin-1可促进侵袭和迁移。此外，Talin-2可能与人肝细胞癌的侵袭和迁移相关。
