Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Hemmat Street (Highway), Next to Milad Tower, Tehran, 14496-14535, Iran.
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
BMC Cancer. 2023 Apr 3;23(1):302. doi: 10.1186/s12885-023-10771-z.
Talin-1 as a component of multi-protein adhesion complexes plays a role in tumor formation and migration in various malignancies. This study investigated Talin-1 in protein levels as a potential prognosis biomarker in skin tumors.
Talin-1 was evaluated in 106 skin cancer (33 melanomas and 73 non-melanomas skin cancer (NMSC)) and 11 normal skin formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemical technique on tissue microarrays (TMAs). The association between the expression of Talin-1 and clinicopathological parameters, as well as survival outcomes, were assessed.
Our findings from data minings through bioinformatics tools indicated dysregulation of Talin-1 in mRNA levels for skin cancer samples. In addition, there was a statistically significant difference in Talin-1 expression in terms of intensity of staining, percentage of positive tumor cells, and H-score in melanoma tissues compared to NMSC (P = 0.001, P < 0.001, and P < 0.001, respectively). Moreover, high cytoplasmic expression of Talin-1 was found to be associated with significantly advanced stages (P = 0.024), lymphovascular invasion (P = 0.023), and recurrence (P = 0.006) in melanoma cancer tissues. Our results on NMSC showed a statistically significant association between high intensity of staining and the poor differentiation (P = 0.044). No significant associations were observed between Talin-1 expression levels and survival outcomes of melanoma and NMSC patients.
Our observations showed that higher expression of Talin1 in protein level may be significantly associated with more aggressive tumor behavior and advanced disease in patients with skin cancer. However, further studies are required to find the mechanism of action of Talin-1 in skin cancers.
Talin-1 作为多蛋白黏附复合物的一个组成部分，在各种恶性肿瘤的肿瘤形成和迁移中发挥作用。本研究探讨了 Talin-1 在蛋白质水平上作为皮肤肿瘤潜在预后生物标志物的作用。
使用组织微阵列（TMA）上的免疫组织化学技术，在 106 例皮肤癌（33 例黑色素瘤和 73 例非黑色素瘤皮肤癌（NMSC））和 11 例正常皮肤福尔马林固定石蜡包埋（FFPE）组织样本中评估 Talin-1。评估 Talin-1 的表达与临床病理参数以及生存结果之间的关系。
通过生物信息学工具进行的数据挖掘结果表明，皮肤癌样本中 Talin-1 的 mRNA 水平失调。此外，与 NMSC 相比，黑色素瘤组织中 Talin-1 表达的染色强度、阳性肿瘤细胞百分比和 H 评分存在统计学差异（P = 0.001，P < 0.001，和 P < 0.001，分别）。此外，发现黑色素瘤组织中细胞质高表达 Talin-1 与明显的晚期阶段（P = 0.024）、淋巴血管侵犯（P = 0.023）和复发（P = 0.006）显著相关。我们在 NMSC 上的结果表明，高强度染色与低分化之间存在统计学显著相关性（P = 0.044）。在黑色素瘤和 NMSC 患者的生存结果中未观察到 Talin-1 表达水平与生存结果之间存在显著相关性。
我们的观察结果表明，Talin1 蛋白水平的高表达可能与皮肤癌患者更具侵袭性的肿瘤行为和晚期疾病显著相关。然而，需要进一步的研究来发现 Talin-1 在皮肤癌中的作用机制。
