Ning A L
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1989 Apr;11(2):129-31.
The expression of phenotypes of cultured cancer cells might be associated with cancer cell differentiation. By using the electron microscope and monoclonal antibodies against cytokeratins, PKK1, PKK2 and PKK3, the expression of cytokeratins and tonofilaments of normal embryonic esophageal epithelial cells and esophageal cancer cells were studied. Both PKK1 and PKK2 cytokeratins were detected in embryonic esophageal and esophageal cancer cells, but PKK3 was not present. This indicated that these were squamous but not glandular epithelial cells. Also, a much greater degree of expression of PKK1 and PKK2, tonofilaments and desmosomes was observed in differentiated embryonic squamous esophageal epithelial cells and in DMSO or retinamide induced differentiated esophageal cancer cells than in undifferentiated esophageal cancer cells.
培养的癌细胞表型的表达可能与癌细胞分化有关。通过使用电子显微镜和抗细胞角蛋白、PKK1、PKK2和PKK3的单克隆抗体，研究了正常胚胎食管上皮细胞和食管癌细胞中细胞角蛋白和张力丝的表达。PKK1和PKK2细胞角蛋白在胚胎食管和食管癌细胞中均被检测到，但PKK3不存在。这表明这些是鳞状上皮细胞而非腺上皮细胞。此外，与未分化的食管癌细胞相比，在分化的胚胎鳞状食管上皮细胞以及二甲基亚砜（DMSO）或视黄酰胺诱导分化的食管癌细胞中，观察到PKK1和PKK2、张力丝和桥粒的表达程度要高得多。
