Gratama J W, Langelaar R A, Oosterveer M A, van der Linden J A, den Ouden-Noordermeer A, Naipal A M, Visser J W, de Gast G C, Tanke H J
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
Clin Exp Immunol. 1989 Aug;77(2):245-51.
A characteristic of active cytomegalovirus (CMV) infection is its suppressive effect on in vitro assays of immune function. The expression of CD11b by the Cd4+ and Cd8+ lymphocytes allows the identification of subsets with distinct regulatory functions of pokeweed mitogen (PWM) induced B cell differentiation. In order to relate that result with our previous observation that CMV carriers have significantly increased numbers of CD4+, HNK1+ and CD8+, HNK1+ lymphocytes in their peripheral blood compared with non-carriers, we performed a three-colour flow cytometric analysis of the co-expression of Cd11b and HNK1 by CD4+ and CD8+ lymphocytes obtained from 27 CMV carriers and 42 non-carriers. The differences between CMV carriers and non-carriers were significant for the CD4+, HNK1+ lymphocytes (median [5th and 95th percentiles], 59 [18 and 123 versus 24/7 and 73 per mm3, respectively; P less than 0.001) and CD8+, HNK1+ lymphocytes (59 [18 259] versus 52 [23 and 139] per mm3; P less than 0.001), but not for the CD4+, CD11b+ lymphocytes (59 [18 and 135] versus 52 [17 and 104] per mm3) and the CD8+, CD11b+ lymphocytes (85 [34 and 293] versus 82 [21 and 248] per mm3). The CD4+, HNK1+ and CD8+, HNK1+ lymphocytes that were increased in CMV carriers compared with non-carriers included mostly CD11b-, but also CD11b+ lymphocytes. After sorting CD4+ and CD8+ lymphocytes for four CMV carriers into HNK1+ and HNK1- fractions, we analyzed their regulatory functions on PWM-driven B cell Helper function to PWM-driven B cell differentiation was exclusively associated with the CD4+, HNK1- lymphocytes; the CD4+, HNK1+ generally did not show helper or suppressor activity in this assay. Both CD8+, HNK1+ and CD8+, HNK1- lymphocytes showed suppressor activity. Thus, the NHK1 marker does not constitute a phenotypical correlate for suppressor cells of PWM-driven B-cell differentiation.
活动性巨细胞病毒（CMV）感染的一个特点是其对免疫功能体外检测有抑制作用。通过Cd4+和Cd8+淋巴细胞上CD11b的表达，可以识别出对美洲商陆有丝分裂原（PWM）诱导的B细胞分化具有不同调节功能的亚群。为了将该结果与我们之前的观察结果联系起来，即与非携带者相比，CMV携带者外周血中CD4+、HNK1+和CD8+、HNK1+淋巴细胞数量显著增加，我们对从27名CMV携带者和42名非携带者获得的CD4+和CD8+淋巴细胞中Cd11b和HNK1的共表达进行了三色流式细胞术分析。CMV携带者和非携带者之间，CD4+、HNK1+淋巴细胞（中位数[第5和第95百分位数]，分别为59[18和123]与24/7和73每立方毫米；P小于0.001）和CD8+、HNK1+淋巴细胞（59[18至259]与52[23和139]每立方毫米；P小于0.001）差异显著，但CD4+、CD11b+淋巴细胞（59[18和135]与52[17和104]每立方毫米）和CD8+、CD11b+淋巴细胞（85[34和293]与82[21和248]每立方毫米）差异不显著。与非携带者相比，CMV携带者中增加的CD4+、HNK1+和CD8+、HNK1+淋巴细胞大多为CD11b-，但也有CD11b+淋巴细胞。将4名CMV携带者的CD4+和CD8+淋巴细胞分选成HNK1+和HNK1-亚群后，我们分析了它们对PWM驱动的B细胞的调节功能。对PWM驱动的B细胞分化的辅助功能仅与CD4+、HNK1-淋巴细胞相关；在该检测中，CD4+、HNK1+通常不显示辅助或抑制活性。CD8+、HNK1+和CD8+、HNK1-淋巴细胞均显示抑制活性。因此，NHK1标记物并不构成PWM驱动的B细胞分化抑制细胞的表型相关物。
