CD4+和CD8+淋巴细胞亚群与巨细胞病毒携带状态的表型研究及其与商陆有丝分裂原诱导的B淋巴细胞分化的相关性

Phenotypic study of CD4+ and CD8+ lymphocyte subsets in relation to cytomegalovirus carrier status and its correlate with pokeweed mitogen-induced B lymphocyte differentiation.

作者信息

Gratama J W, Langelaar R A, Oosterveer M A, van der Linden J A, den Ouden-Noordermeer A, Naipal A M, Visser J W, de Gast G C, Tanke H J

机构信息

Department of Immunohaematology, University Hospital, Leiden, The Netherlands.

出版信息

Clin Exp Immunol. 1989 Aug;77(2):245-51.

PMID:2476271

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1542001/

Abstract

A characteristic of active cytomegalovirus (CMV) infection is its suppressive effect on in vitro assays of immune function. The expression of CD11b by the Cd4+ and Cd8+ lymphocytes allows the identification of subsets with distinct regulatory functions of pokeweed mitogen (PWM) induced B cell differentiation. In order to relate that result with our previous observation that CMV carriers have significantly increased numbers of CD4+, HNK1+ and CD8+, HNK1+ lymphocytes in their peripheral blood compared with non-carriers, we performed a three-colour flow cytometric analysis of the co-expression of Cd11b and HNK1 by CD4+ and CD8+ lymphocytes obtained from 27 CMV carriers and 42 non-carriers. The differences between CMV carriers and non-carriers were significant for the CD4+, HNK1+ lymphocytes (median [5th and 95th percentiles], 59 [18 and 123 versus 24/7 and 73 per mm3, respectively; P less than 0.001) and CD8+, HNK1+ lymphocytes (59 [18 259] versus 52 [23 and 139] per mm3; P less than 0.001), but not for the CD4+, CD11b+ lymphocytes (59 [18 and 135] versus 52 [17 and 104] per mm3) and the CD8+, CD11b+ lymphocytes (85 [34 and 293] versus 82 [21 and 248] per mm3). The CD4+, HNK1+ and CD8+, HNK1+ lymphocytes that were increased in CMV carriers compared with non-carriers included mostly CD11b-, but also CD11b+ lymphocytes. After sorting CD4+ and CD8+ lymphocytes for four CMV carriers into HNK1+ and HNK1- fractions, we analyzed their regulatory functions on PWM-driven B cell Helper function to PWM-driven B cell differentiation was exclusively associated with the CD4+, HNK1- lymphocytes; the CD4+, HNK1+ generally did not show helper or suppressor activity in this assay. Both CD8+, HNK1+ and CD8+, HNK1- lymphocytes showed suppressor activity. Thus, the NHK1 marker does not constitute a phenotypical correlate for suppressor cells of PWM-driven B-cell differentiation.

摘要

活动性巨细胞病毒（CMV）感染的一个特点是其对免疫功能体外检测有抑制作用。通过Cd4+和Cd8+淋巴细胞上CD11b的表达，可以识别出对美洲商陆有丝分裂原（PWM）诱导的B细胞分化具有不同调节功能的亚群。为了将该结果与我们之前的观察结果联系起来，即与非携带者相比，CMV携带者外周血中CD4+、HNK1+和CD8+、HNK1+淋巴细胞数量显著增加，我们对从27名CMV携带者和42名非携带者获得的CD4+和CD8+淋巴细胞中Cd11b和HNK1的共表达进行了三色流式细胞术分析。CMV携带者和非携带者之间，CD4+、HNK1+淋巴细胞（中位数[第5和第95百分位数]，分别为59[18和123]与24/7和73每立方毫米；P小于0.001）和CD8+、HNK1+淋巴细胞（59[18至259]与52[23和139]每立方毫米；P小于0.001）差异显著，但CD4+、CD11b+淋巴细胞（59[18和135]与52[17和104]每立方毫米）和CD8+、CD11b+淋巴细胞（85[34和293]与82[21和248]每立方毫米）差异不显著。与非携带者相比，CMV携带者中增加的CD4+、HNK1+和CD8+、HNK1+淋巴细胞大多为CD11b-，但也有CD11b+淋巴细胞。将4名CMV携带者的CD4+和CD8+淋巴细胞分选成HNK1+和HNK1-亚群后，我们分析了它们对PWM驱动的B细胞的调节功能。对PWM驱动的B细胞分化的辅助功能仅与CD4+、HNK1-淋巴细胞相关；在该检测中，CD4+、HNK1+通常不显示辅助或抑制活性。CD8+、HNK1+和CD8+、HNK1-淋巴细胞均显示抑制活性。因此，NHK1标记物并不构成PWM驱动的B细胞分化抑制细胞的表型相关物。