Lin Chi Chen, Chen Hua Han, Chen Yu Kuo, Chang Hung Chia, Lin Ping Yi, Pan I-Hong, Chen Der-Yuan, Chen Chuan Mu, Lin Su Yi
Institute of Biomedical Science, National Chung-Hsing University, Taichung 402, Taiwan.
Department of Food Science, National Penghu University of Science and Technology, Makung City, Penghu Hsien 880, Taiwan.
Molecules. 2014 Apr 23;19(4):5325-47. doi: 10.3390/molecules19045325.
This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-kB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination.
本研究展示了米糠阿魏酰化低聚糖（FOs）对小鼠骨髓来源树突状细胞（BMDCs）的影响以及介导这些影响的潜在途径。我们发现，FOs可诱导树突状细胞的表型成熟，表现为CD40、CD80/CD86和MHC-I/II分子表达增加。FOs能有效诱导来自具有正常Toll样受体4（TLR-4）或TLR-2的C3H/HeN或C57BL/6小鼠的树突状细胞成熟，但不能诱导来自TLR4或TLR2突变小鼠的树突状细胞成熟。FOs的作用机制可能是通过增加细胞外调节蛋白激酶（ERK）、p38和c-Jun氨基末端激酶（JNK）丝裂原活化蛋白激酶（MAPKs）的磷酸化以及增加核因子-κB（NF-κB）活性来介导，这些都是TLR-4和TLR-2下游的重要信号分子。这些数据表明，FOs通过TLR-4和/或TLR-2诱导树突状细胞成熟，并且FOs可能对肿瘤或病毒感染具有潜在疗效，或者代表一种可用于免疫治疗和疫苗接种的候选佐剂方法。
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