Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Centre for the Study of Social Insects, UNESP - São Paulo State University, Rio Claro, SP, Brazil.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2364-2369. doi: 10.1099/ijs.0.061499-0. Epub 2014 Apr 24.
Seven apiculate yeast strains that were isolated from the flowers of Syphocampylus corymbiferus Pohl in Brazil are genetically, morphologically and phenotypically distinct from recognized species of the genera Hanseniaspora and Kloeckera. Genetic discontinuities between the novel strains and their closest relatives were found using a networking approach based on the concatenated sequences of the rRNA gene (internal transcribed spacer and D1/D2 of the LSU), and the protein-coding genes for actin and translation elongation factor-1α. Phylogenetic analysis based on the rRNA and the actin gene placed the novel species represented by the strains in close relationship to Hanseniaspora meyeri and Hanseniaspora clermontiae. PCR fingerprinting with microsatellite primers confirmed the genetic heterogeneity of the novel species. The name Hanseniaspora nectarophila sp. nov. is proposed, with UFMG POG a.1(T) ( = ZIM 2311(T) = CBS 13383(T)) as the type strain; MycoBank no. MB807210. As the current description of the genus does not allow the presence of multilateral budding, an emended diagnosis of the genus Hanseniaspora Zikes is proposed.
从巴西的 Syphocampylus corymbiferus Pohl 花中分离出来的七株具尖顶的酵母菌株，在遗传上、形态上和表型上与公认的 Hanseniaspora 和 Kloeckera 属的种不同。利用基于 rRNA 基因（内部转录间隔区和 LSU 的 D1/D2）和肌动蛋白及翻译延伸因子 1α 的编码蛋白基因的连锁分析方法，发现了新菌株与其最接近的亲缘之间存在遗传不连续性。基于 rRNA 和肌动蛋白基因的系统发育分析将由这些菌株代表的新种与 Hanseniaspora meyeri 和 Hanseniaspora clermontiae 密切相关。用微卫星引物进行的 PCR 指纹分析证实了新种的遗传异质性。提议使用 UFMG POG a.1(T)（=ZIM 2311(T)=CBS 13383(T)）作为模式菌株，命名为 Hanseniaspora nectarophila sp. nov.；MycoBank 编号 MB807210。由于目前对该属的描述不允许存在多边出芽，因此提议对 Hanseniaspora Zikes 属进行修正诊断。
