Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia.
Laboratorio de Microbiología Aplicada y Biotecnología, Instituto de Investigaciones en Biodiversidad y Medio-ambiente, Consejo Nacional de Investigaciones, Científicas y Técnicas (CONICET)-Universidad Nacional del Comahue, Bariloche, Argentina.
PLoS One. 2019 Jan 30;14(1):e0210792. doi: 10.1371/journal.pone.0210792. eCollection 2019.
A novel yeast species was isolated from the sugar-rich stromata of Cyttaria hariotii collected from two different Nothofagus tree species in the Andean forests of Patagonia, Argentina. Phylogenetic analyses of the concatenated sequence of the rRNA gene sequences and the protein-coding genes for actin and translational elongation factor-1α indicated that the novel species belongs to the genus Hanseniaspora. De novo genome assembly of the strain CRUB 1928T yielded a 10.2-Mbp genome assembly predicted to encode 4452 protein-coding genes. The genome sequence data were compared to the genomes of other Hanseniaspora species using three different methods, an alignment-free distance measure, Kr, and two model-based estimations of DNA-DNA homology values, of which all provided indicative values to delineate species of Hanseniaspora. Given its potential role in a rare indigenous alcoholic beverage in which yeasts ferment sugars extracted from the stromata of Cytarria sp., we searched for the genes that may suggest adaptation of novel Hanseniaspora species to fermenting communities. The SSU1-like gene encoding a sulfite efflux pump, which, among Hanseniaspora, is present only in close relatives to the new species, was detected and analyzed, suggesting that this gene might be one factor that characterizes this novel species. We also discuss several candidate genes that likely underlie the physiological traits used for traditional taxonomic identification. Based on these results, a novel yeast species with the name Hanseniaspora gamundiae sp. nov. is proposed with CRUB 1928T (ex-types: ZIM 2545T = NRRL Y-63793T = PYCC 7262T; MycoBank number MB 824091) as the type strain. Furthermore, we propose the transfer of the Kloeckera species, K. hatyaiensis, K. lindneri and K. taiwanica to the genus Hanseniaspora as Hanseniaspora hatyaiensis comb. nov. (MB 828569), Hanseniaspora lindneri comb. nov. (MB 828566) and Hanseniaspora taiwanica comb. nov. (MB 828567).
从阿根廷巴塔哥尼亚安第斯森林中两种不同的诺福克南洋杉树上采集到的富含糖分的 Cyttaria hariotii 糖蜜中分离到一株新型酵母。rRNA 基因序列和肌动蛋白及翻译延伸因子-1α 的蛋白编码基因的串联序列的系统发育分析表明，该新型物种属于 Hanseniaspora 属。该菌株 CRUB 1928T 的从头基因组组装产生了一个 10.2-Mbp 的基因组组装，预计编码 4452 个蛋白编码基因。使用三种不同的方法，即无比对距离度量 Kr 和两种基于模型的 DNA-DNA 同源性值估计，将基因组序列数据与其他 Hanseniaspora 物种的基因组序列进行比较，所有这些方法都提供了指示值来划定 Hanseniaspora 物种。鉴于其在一种罕见的本土酒精饮料中的潜在作用，酵母从 Cytarria sp. 的糖蜜中发酵糖，我们搜索了可能表明新型 Hanseniaspora 物种适应发酵群落的基因。检测到并分析了编码亚硫酸盐外排泵的 SSU1 样基因，该基因在 Hanseniaspora 中仅存在于与新物种密切相关的亲缘关系中，表明该基因可能是该新型物种的一个特征因素。我们还讨论了几个可能为传统分类鉴定所依据的生理特征的候选基因。基于这些结果，提出了一个新的酵母物种，命名为 Hanseniaspora gamundiae sp. nov.，其模式菌株为 CRUB 1928T（ex-types：ZIM 2545T = NRRL Y-63793T = PYCC 7262T；MycoBank 编号 MB 824091）。此外，我们建议将 Kloeckera 物种，K. hatyaiensis、K. lindneri 和 K. taiwanica 转移到 Hanseniaspora 属，分别命名为 Hanseniaspora hatyaiensis comb. nov.（MB 828569）、Hanseniaspora lindneri comb. nov.（MB 828566）和 Hanseniaspora taiwanica comb. nov.（MB 828567）。
