Hou Xiaodong, Zhang Tao, Cao Amin
Lab of Materials Science, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China.
J Biomed Mater Res B Appl Biomater. 2015 Jan;103(1):52-61. doi: 10.1002/jbm.b.33190. Epub 2014 Apr 25.
In the present work, new amphiphilic macroporous polymeric adsorbent (AMPA) membranes for LDL-apheresis were prepared by (60)Co γ-ray irradiation-induced grafting copolymerization of polypropylene (PP) nonwoven fabric with acrylic acid, followed by bonding cholesterol through linkers of different length. The new AMPA membranes were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, scanning electron microscope (SEM), and contact angle microscopy. Static adsorption and hemo-perfusion tests show these new adsorbents could efficiently remove LDL from human plasma. Meanwhile, the AMPA displayed good adsorption capacity for triglyceride (TG) as well. The static adsorption performance of the AMPA membranes depends on the length of linker. In addition, a balance between the amount of bonded cholesterol and remaining carboxyl group was found necessary to reach the optimal adsorption performance. The best result was achieved by the AMPA membrane PA15C6-3, by which 62.8 ± 3.8 μg of LDL-C, 16.5 ± 0.71 μg of HDL-C, 132.4 ± 3.0 μg of TG are removed from human plasma per square centimeter.
在本工作中，通过（60）Coγ射线辐照诱导聚丙烯（PP）无纺布与丙烯酸接枝共聚，随后通过不同长度的连接体连接胆固醇，制备了用于低密度脂蛋白去除术的新型两亲性大孔聚合物吸附剂（AMPA）膜。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、扫描电子显微镜（SEM）和接触角显微镜对新型AMPA膜进行了表征。静态吸附和血液灌注试验表明，这些新型吸附剂能够有效地从人血浆中去除低密度脂蛋白。同时，AMPA对甘油三酯（TG）也表现出良好的吸附能力。AMPA膜的静态吸附性能取决于连接体的长度。此外，发现结合胆固醇的量与剩余羧基之间的平衡对于达到最佳吸附性能是必要的。AMPA膜PA15C6-3取得了最佳结果，每平方厘米可从人血浆中去除62.8±3.8μg低密度脂蛋白胆固醇、16.5±0.71μg高密度脂蛋白胆固醇、132.4±3.0μg甘油三酯。
