Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.
School of Ophthalmology & Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
J Mater Chem B. 2022 Jun 29;10(25):4856-4866. doi: 10.1039/d2tb00291d.
Removal of low-density lipoprotein (LDL) from hyperlipemia patients' blood represents an effective approach to prevent the progression of atherosclerotic cardiovascular disease. Based on the LDL structural characteristics and intermolecular interactions, a tailored nano-adsorbent (FeO@SiO@PAA-PE) was prepared aimed at the removal of LDL from hyperlipemia serum with high selectivity. The core-shell structured magnetic nanoparticles were embedded in an amphiphilic layer composed of hydrophilic poly(acrylic acid) and lipophilic phospholipids to provide multifunctional binding for LDL particles. The results of dynamic light scattering, water contact angle and zeta-potential measurements, thermal gravimetric analysis, and X-ray photoelectron spectroscopy together with Fourier transform infrared spectroscopy confirmed the core-shell structured nanoparticles bearing amphiphilic poly acrylic acid and phospholipid molecules. Because of the superior electronegativity of the functional layer, the nano-adsorbent demonstrated favorable adsorption selectivity against high-density lipoprotein, which possesses a similar structure to LDL but has a cardio-protective function in the human body. The respective adsorption capacity of FeO@SiO@PAA-PE towards LDL, total cholesterol and triglycerides reached up to 6.26 mg g, 8.41 mg g and 9.19 mg g, which was 7.03, 9.45 and 10.32 times that towards HDL (0.89 mg g). The kinetic and isothermal studies revealed that multiple interactions containing both physical and chemical adsorption occurred in the binding procedure between LDL and FeO@SiO@PAA-PE, and chemical adsorption may play a more predominant role in LDL adsorption. The nano-adsorbent also had negligible effects on blood cells, and possessed satisfactory recyclability, low cytotoxicity and hemolysis ratios, indicating its good application prospects as a hemoperfusion adsorbent in the treatment of hyperlipidaemia.
从高脂血症患者血液中去除低密度脂蛋白（LDL）是预防动脉粥样硬化性心血管疾病进展的有效方法。基于 LDL 的结构特征和分子间相互作用，设计了一种定制的纳米吸附剂（FeO@SiO@PAA-PE），旨在从高脂血症血清中高选择性地去除 LDL。核壳结构的磁性纳米颗粒被嵌入由亲水性聚丙烯酸和疏水性磷脂组成的两亲层中，为 LDL 颗粒提供多功能结合。动态光散射、水接触角和 zeta 电位测量、热重分析、X 射线光电子能谱和傅里叶变换红外光谱的结果共同证实了具有两亲性聚丙烯酸和磷脂分子的核壳结构纳米颗粒。由于功能层的卓越电负性，纳米吸附剂对具有与 LDL 相似结构但在人体中具有心脏保护功能的高密度脂蛋白（HDL）表现出良好的吸附选择性。FeO@SiO@PAA-PE 对 LDL、总胆固醇和甘油三酯的吸附容量分别高达 6.26、8.41 和 9.19mg/g，是对 HDL（0.89mg/g）的 7.03、9.45 和 10.32 倍。动力学和等温研究表明，LDL 和 FeO@SiO@PAA-PE 之间的结合过程中发生了多种包含物理吸附和化学吸附的相互作用，化学吸附可能在 LDL 吸附中起更主要的作用。纳米吸附剂对血细胞几乎没有影响，且具有良好的可回收性、低细胞毒性和溶血率，表明其作为血液灌流吸附剂在高脂血症治疗中具有良好的应用前景。
