Departments of Chemical Engineering, Chemistry & Chemical Biology, and Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4M1 (Canada).
Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6155-8. doi: 10.1002/anie.201403222. Epub 2014 Apr 24.
A simple and inexpensive method is reported for the long-term stabilization of enzymes and other unstable reagents in premeasured quantities in water-soluble tablets (cast, not compressed) made with pullulan, a nonionic polysaccharide that forms an oxygen impermeable solid upon drying. The pullulan tablets dissolve in aqueous solutions in seconds, thereby facilitating the easy execution of bioassays at remote sites with no need for special reagent handling and liquid pipetting. This approach is modular in nature, thus allowing the creation of individual tablets for enzymes and their substrates. Proof-of-principle demonstrations include a Taq polymerase tablet for DNA amplification through PCR and a pesticide assay kit consisting of separate tablets for acetylcholinesterase and its chromogenic substrate, indoxyl acetate, both of which are highly unstable. The encapsulated reagents remain stable at room temperature for months, thus enabling the room-temperature shipping and storage of bioassay components.
报道了一种简单且廉价的方法，用于将酶和其他不稳定试剂长期稳定在水溶性片剂（铸造而非压缩）中，这些片剂由出芽短梗霉多糖制成，这是一种非离子多糖，在干燥时形成不可渗透氧气的固体。出芽短梗霉多糖片剂在水溶液中可在数秒内溶解，从而便于在偏远地点轻松进行生物测定，无需特殊试剂处理和液体移液。该方法具有模块性，因此可以为酶及其底物创建单独的片剂。原理验证演示包括用于通过 PCR 进行 DNA 扩增的 Taq 聚合酶片剂，以及由乙酰胆碱酯酶及其显色底物吲哚乙酸酯组成的单独片剂的农药测定试剂盒，这两者都极不稳定。包封的试剂在室温下可稳定数月，从而能够在室温下运输和储存生物测定组件。
