Döpel S H, Porstmann T, Henklein P, von Baehr R
Department of Medicine, Humboldt University Berlin, G.D.R.
J Virol Methods. 1989 Aug;25(2):167-77. doi: 10.1016/0166-0934(89)90030-x.
Peptides of HIV sequences are significant for antibody screening systems, and because of the limited number of amino acids they have to represent immunodominant regions of the virus proteins in order to maintain sensitivity. We detected, in a region of the outside loop of the transmembrane protein gp41 of the human immunodeficiency virus HIV-1 (amino acid 586-620), two immunodominant sequences which are distinct from each other. Whereas in the first immunodominant region the sensitivity and specificity of ELISA were inadequate, a neighbouring region is well suited for use as antigen for an anti-HIV screening ELISA.
HIV序列的肽段对于抗体筛选系统具有重要意义，并且由于它们的氨基酸数量有限，必须代表病毒蛋白的免疫显性区域以保持敏感性。我们在人类免疫缺陷病毒HIV-1跨膜蛋白gp41外环的一个区域（氨基酸586 - 620）中检测到两个彼此不同的免疫显性序列。虽然在第一个免疫显性区域，ELISA的敏感性和特异性不足，但相邻区域非常适合用作抗HIV筛选ELISA的抗原。
