用于脑钠肽的DNA适配体-磁珠捕获电化学发光夹心分析法的初步开发
Preliminary Development of a DNA Aptamer-Magnetic Bead Capture Electrochemiluminescence Sandwich Assay for Brain Natriuretic Peptide.
作者信息
Bruno John G, Richarte Alicia M, Phillips Taylor
机构信息
Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229.
出版信息
Microchem J. 2014 Jul 1;115:32-38. doi: 10.1016/j.microc.2014.02.003.
Abstract
Fifty-two candidate DNA aptamer sequences were selected for binding to the cardiovascular biomarker B-type or brain natriuretic peptide (BNP). Candidate aptamers were screened to rank their relative affinities against BNP by an aptamer-based ELISA-like aptamer microplate assay (ELASA). The highest affinity aptamers from ELASA screening were also paired in all possible combinations and screened for electrochemiluminescence (ECL) assay potential in capture aptamer-magnetic bead and ruthenium trisbipyridine (Ru(bpy))-reporter aptamer sandwich formats. The top ECL sandwich combinations utilized the same aptamer pair in either capture or reporting roles with nanogram to low picogram per mL levels of detection even in 50% human serum. ECL assay sensitivity and linearity even in 50% human serum suggest that the aptamer-based assay is at least comparable to other reported immunoassays for BNP.
摘要
选择了52个候选DNA适配体序列用于与心血管生物标志物B型或脑钠肽（BNP）结合。通过基于适配体的酶联免疫吸附测定样适配体微孔板分析（ELASA）筛选候选适配体，以对它们与BNP的相对亲和力进行排名。来自ELASA筛选的具有最高亲和力的适配体也以所有可能的组合配对，并针对捕获适配体-磁珠和三联吡啶钌（Ru(bpy)）-报告适配体夹心形式的电化学发光（ECL）分析潜力进行筛选。即使在50%人血清中，顶级ECL夹心组合在捕获或报告角色中使用相同的适配体对，检测水平可达每毫升纳克至低皮克。即使在50%人血清中，ECL分析的灵敏度和线性表明基于适配体的分析至少与其他报道的BNP免疫分析相当。
相似文献
1
Preliminary Development of a DNA Aptamer-Magnetic Bead Capture Electrochemiluminescence Sandwich Assay for Brain Natriuretic Peptide.用于脑钠肽的DNA适配体-磁珠捕获电化学发光夹心分析法的初步开发
Microchem J. 2014 Jul 1;115:32-38. doi: 10.1016/j.microc.2014.02.003.
2
Integration of multiple computer modeling software programs for characterization of a brain natriuretic peptide sandwich DNA aptamer complex.整合多个计算机建模软件程序，用于表征脑利钠肽三明治 DNA 适体复合物。
J Mol Recognit. 2019 Dec;32(12):e2809. doi: 10.1002/jmr.2809. Epub 2019 Aug 16.
3
Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods.磁珠在通过电化学发光和酶法筛选与检测生物毒素适配体中的应用。
Biotechniques. 2002 Jan;32(1):178-80, 182-3. doi: 10.2144/02321dd04.
4
Development, screening, and analysis of DNA aptamer libraries potentially useful for diagnosis and passive immunity of arboviruses.对可能用于虫媒病毒诊断和被动免疫的DNA适配体文库的开发、筛选及分析。
BMC Res Notes. 2012 Nov 13;5:633. doi: 10.1186/1756-0500-5-633.
5
A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein.基于新型适体的免疫磁电化学发光法定量检测蛋白质
Biosens Bioelectron. 2010 Sep 15;26(1):285-8. doi: 10.1016/j.bios.2010.06.028. Epub 2010 Jun 25.
6
Isolation of a novel DNA aptamer against LipL32 as a potential diagnostic agent for the detection of pathogenic Leptospira.分离针对 LipL32 的新型 DNA 适体作为一种潜在的诊断试剂用于检测致病性钩端螺旋体。
Biotechnol J. 2023 Mar;18(3):e2200418. doi: 10.1002/biot.202200418. Epub 2022 Dec 8.
7
Further characterization and independent validation of a DNA aptamer-quantum dot-based magnetic sandwich assay for Campylobacter.基于DNA适配体-量子点的弯曲杆菌磁性夹心分析方法的进一步表征及独立验证
Folia Microbiol (Praha). 2017 Nov;62(6):485-490. doi: 10.1007/s12223-017-0520-0. Epub 2017 Mar 24.
8
Gold nanoparticle-streptavidin conjugates for rapid and efficient screening of aptamer function in lateral flow sensors using novel CD4-binding aptamers identified through Crossover-SELEX.金纳米颗粒-链霉亲和素缀合物用于使用通过交叉SELEX鉴定的新型CD4结合适体在侧流传感器中快速高效地筛选适体功能。
Analyst. 2020 Aug 7;145(15):5180-5193. doi: 10.1039/d0an00634c. Epub 2020 Jun 22.
9
In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detection.通过电化学发光检测对炭疽芽孢杆菌孢子进行DNA适配体的体外筛选。
Biosens Bioelectron. 1999 May 31;14(5):457-64. doi: 10.1016/s0956-5663(99)00028-7.
10
A signal-on electrochemiluminescence aptamer biosensor for the detection of ultratrace thrombin based on junction-probe.基于结探针的用于检测痕量凝血酶的信号放大电化学发光适体生物传感器。
Biosens Bioelectron. 2011 Jan 15;26(5):2645-50. doi: 10.1016/j.bios.2010.11.028. Epub 2010 Nov 27.
引用本文的文献
1
Successes and Failures of Static Aptamer-Target 3D Docking Models.静态适体-靶标 3D 对接模型的成功与失败。
Int J Mol Sci. 2022 Nov 19;23(22):14410. doi: 10.3390/ijms232214410.
2
Aptamers Targeting Cardiac Biomarkers as an Analytical Tool for the Diagnostics of Cardiovascular Diseases: A Review.靶向心脏生物标志物的适体作为心血管疾病诊断的分析工具：综述
Biomedicines. 2022 May 6;10(5):1085. doi: 10.3390/biomedicines10051085.
3
Engineered Aptamers for Enhanced COVID-19 Theranostics.用于增强 COVID-19 诊疗的工程适体
Cell Mol Bioeng. 2021 Jan 15;14(3):209-221. doi: 10.1007/s12195-020-00664-7. eCollection 2021 Jun.
4
Diagnosing Perioperative Cardiovascular Risks in Noncardiac Surgery Patients.诊断非心脏手术患者围手术期心血管风险
J Anal Methods Chem. 2019 Aug 25;2019:6097375. doi: 10.1155/2019/6097375. eCollection 2019.
5
Integration of multiple computer modeling software programs for characterization of a brain natriuretic peptide sandwich DNA aptamer complex.整合多个计算机建模软件程序，用于表征脑利钠肽三明治 DNA 适体复合物。
J Mol Recognit. 2019 Dec;32(12):e2809. doi: 10.1002/jmr.2809. Epub 2019 Aug 16.
6
Beacons Contribute Valuable Empirical Information to Theoretical 3-D Aptamer-Peptide Binding.信标为理论的 3D 适体-肽结合提供有价值的经验信息。
J Fluoresc. 2019 May;29(3):711-717. doi: 10.1007/s10895-019-02380-6. Epub 2019 May 1.
7
Electrochemical Aptamer-Based Biosensors for the Detection of Cardiac Biomarkers.用于检测心脏生物标志物的基于电化学适配体的生物传感器。
ACS Omega. 2018 Sep 30;3(9):12010-12018. doi: 10.1021/acsomega.8b01558. Epub 2018 Sep 26.
8
Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity.增强适配体敏感性和特异性的简单方法与合理设计
Front Mol Biosci. 2018 May 14;5:41. doi: 10.3389/fmolb.2018.00041. eCollection 2018.
9
Gold nanoparticle based photometric determination of tobramycin by using new specific DNA aptamers.基于金纳米颗粒并利用新型特异性DNA适配体对妥布霉素进行光度测定
Mikrochim Acta. 2017 Dec 2;185(1):4. doi: 10.1007/s00604-017-2568-6.
10
A review of electrogenerated chemiluminescent biosensors for assays in biological matrices.用于生物基质分析的电化学发光生物传感器综述。
Bioanalysis. 2016 Oct;8(19):2071-89. doi: 10.4155/bio-2016-0178. Epub 2016 Sep 9.
本文引用的文献
1
Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform.基于表面等离子体共振平台的心脏生物标志物的超灵敏和超宽检测范围。
Anal Chem. 2014 Jan 7;86(1):814-9. doi: 10.1021/ac4033565. Epub 2013 Dec 20.
2
A review of therapeutic aptamer conjugates with emphasis on new approaches.治疗性适体偶联物的综述，重点介绍新方法。
Pharmaceuticals (Basel). 2013 Mar 19;6(3):340-57. doi: 10.3390/ph6030340.
3
Development, screening, and analysis of DNA aptamer libraries potentially useful for diagnosis and passive immunity of arboviruses.对可能用于虫媒病毒诊断和被动免疫的DNA适配体文库的开发、筛选及分析。
BMC Res Notes. 2012 Nov 13;5:633. doi: 10.1186/1756-0500-5-633.
4
DNA aptamer beacon assay for C-telopeptide and handheld fluorometer to monitor bone resorption.用于 C 端肽的 DNA 适体信标分析和手持式荧光计来监测骨吸收。
J Fluoresc. 2011 Sep;21(5):2021-33. doi: 10.1007/s10895-011-0903-6. Epub 2011 Jun 4.
5
Discrimination of recombinant from natural human growth hormone using DNA aptamers.使用DNA适体鉴别重组人生长激素与天然人生长激素。
J Biomol Tech. 2011 Apr;22(1):27-36.
6
The role of B-type and other natriuretic peptides in health and disease.B型及其他利钠肽在健康与疾病中的作用。
Perm J. 2008 Fall;12(4):32-43. doi: 10.7812/TPP/08-019.
7
Direct comparison of serial B-type natriuretic peptide and NT-proBNP levels for prediction of short- and long-term outcome in acute decompensated heart failure.比较脑利钠肽前体和氨基末端脑利钠肽原水平对急性失代偿性心力衰竭患者短期和长期预后的预测作用。
Crit Care. 2011;15(1):R1. doi: 10.1186/cc9398. Epub 2011 Jan 5.
8
A signal-on electrochemiluminescence aptamer biosensor for the detection of ultratrace thrombin based on junction-probe.基于结探针的用于检测痕量凝血酶的信号放大电化学发光适体生物传感器。
Biosens Bioelectron. 2011 Jan 15;26(5):2645-50. doi: 10.1016/j.bios.2010.11.028. Epub 2010 Nov 27.
9
A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia.一种针对人淋巴瘤和白血病 B 细胞受体的多价 DNA 适体。
Nucleic Acids Res. 2011 Mar;39(6):2458-69. doi: 10.1093/nar/gkq996. Epub 2010 Oct 28.
10
A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein.基于新型适体的免疫磁电化学发光法定量检测蛋白质
Biosens Bioelectron. 2010 Sep 15;26(1):285-8. doi: 10.1016/j.bios.2010.06.028. Epub 2010 Jun 25.
Zotero 插件